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Diltiazem Promotes Regenerative Axon Growth.

ABSTRACT: Axotomy results in permanent loss of function after brain and spinal cord injuries due to the minimal regenerative propensity of the adult central nervous system (CNS). To identify pharmacological enhancers of axon regeneration, 960 compounds were screened for cortical neuron axonal regrowth using an in vitro cortical scrape assay. Diltiazem, verapamil, and bromopride were discovered to facilitate axon regeneration in rat cortical cultures, in the presence of chondroitin sulfate proteoglycans (CSPGs). Diltiazem, an L-type calcium channel blocker (L-CCB), also promotes axon outgrowth in adult primary mouse dorsal root ganglion (DRG) and induced human sensory (iSensory) neurons.

SUBMITTER: Huebner EA

PROVIDER: S-EPMC6424671 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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Diltiazem Promotes Regenerative Axon Growth.

Huebner Eric A EA Budel Stéphane S Jiang Zhaoxin Z Omura Takao T Ho Tammy Szu-Yu TS Barrett Lee L Merkel Janie S JS Pereira Luis M LM Andrews Nick A NA Wang Xingxing X Singh Bhagat B Kapur Kush K Costigan Michael M Strittmatter Stephen M SM Woolf Clifford J CJ

Molecular neurobiology 20180919 6

Axotomy results in permanent loss of function after brain and spinal cord injuries due to the minimal regenerative propensity of the adult central nervous system (CNS). To identify pharmacological enhancers of axon regeneration, 960 compounds were screened for cortical neuron axonal regrowth using an in vitro cortical scrape assay. Diltiazem, verapamil, and bromopride were discovered to facilitate axon regeneration in rat cortical cultures, in the presence of chondroitin sulfate proteoglycans (C ...[more]

PMID: 30232777

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