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Discovery of a Diazo-Forming Enzyme in Cremeomycin Biosynthesis.


ABSTRACT: The molecular architectures and potent bioactivities of diazo-containing natural products have attracted the interest of synthetic and biological chemists. Despite this attention, the biosynthetic enzymes involved in diazo group construction have not been identified. Here, we show that the ATP-dependent enzyme CreM installs the diazo group in cremeomycin via late-stage N-N bond formation using nitrite. This finding should inspire efforts to use diazo-forming enzymes in biocatalysis and synthetic biology as well as enable genome-based discovery of new diazo-containing metabolites.

SUBMITTER: Waldman AJ 

PROVIDER: S-EPMC6425738 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature

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Discovery of a Diazo-Forming Enzyme in Cremeomycin Biosynthesis.

Waldman Abraham J AJ   Balskus Emily P EP  

The Journal of organic chemistry 20180529 14


The molecular architectures and potent bioactivities of diazo-containing natural products have attracted the interest of synthetic and biological chemists. Despite this attention, the biosynthetic enzymes involved in diazo group construction have not been identified. Here, we show that the ATP-dependent enzyme CreM installs the diazo group in cremeomycin via late-stage N-N bond formation using nitrite. This finding should inspire efforts to use diazo-forming enzymes in biocatalysis and synthetic  ...[more]

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