Dataset Information

Proteomic Analysis of the Human Anterior Pituitary Gland.

ABSTRACT: The pituitary function is regulated by a complex system involving the hypothalamus and biological networks within the pituitary. Although the hormones secreted from the pituitary have been well studied, comprehensive analyses of the pituitary proteome are limited. Pituitary proteomics is a field of postgenomic research that is crucial to understand human health and pituitary diseases. In this context, we report here a systematic proteomic profiling of human anterior pituitary gland (adenohypophysis) using high-resolution Fourier transform mass spectrometry. A total of 2164 proteins were identified in this study, of which 105 proteins were identified for the first time compared with high-throughput proteomic-based studies from human pituitary glands. In addition, we identified 480 proteins with secretory potential and 187?N-terminally acetylated proteins. These are the first region-specific data that could serve as a vital resource for further investigations on the physiological role of the human anterior pituitary glands and the proteins secreted by them. We anticipate that the identification of previously unknown proteins in the present study will accelerate biomedical research to decipher their role in functioning of the human anterior pituitary gland and associated human diseases.

SUBMITTER: Yelamanchi SD

PROVIDER: S-EPMC6425986 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature

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Proteomic Analysis of the Human Anterior Pituitary Gland.

Yelamanchi Soujanya D SD Tyagi Ankur A Mohanty Varshasnata V Dutta Pinaki P Korbonits Márta M Chavan Sandip S Advani Jayshree J Madugundu Anil K AK Dey Gourav G Datta Keshava K KK Rajyalakshmi M M Sahasrabuddhe Nandini A NA Chaturvedi Abhishek A Kumar Amit A Das Apabrita Ayan AA Ghosh Dhiman D Jogdand Gajendra M GM Nair Haritha H HH Saini Keshav K Panchal Manoj M Sarvaiya Mansi Ashwinsinh MA Mohanraj Soundappan S SS Sengupta Nabonita N Saxena Priti P Subramani Pradeep Annamalai PA Kumar Pradeep P Akkali Rakhil R Reshma Saraswatipura Vishwabrahmachar SV Santhosh Ramachandran Sarojini RS Rastogi Sangita S Kumar Sudarshan S Ghosh Susanta Kumar SK Irlapati Vamshi Krishna VK Srinivasan Anand A Radotra Bishan Das BD Mathur Premendu P PP Wong G William GW Satishchandra Parthasarathy P Chatterjee Aditi A Gowda Harsha H Bhansali Anil A Pandey Akhilesh A Shankar Susarla K SK Mahadevan Anita A Prasad T S Keshava TSK

Omics : a journal of integrative biology 20181201 12

The pituitary function is regulated by a complex system involving the hypothalamus and biological networks within the pituitary. Although the hormones secreted from the pituitary have been well studied, comprehensive analyses of the pituitary proteome are limited. Pituitary proteomics is a field of postgenomic research that is crucial to understand human health and pituitary diseases. In this context, we report here a systematic proteomic profiling of human anterior pituitary gland (adenohypophy ...[more]

PMID: 30571610

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Project description:To date, very few mass spectrometry (MS)-based proteomics studies are available on the anterior and posterior lobes of the pituitary. In the past, MS-based investigations have focused exclusively on the whole pituitary gland or anterior pituitary lobe. In this study, for the first time, we performed a deep MS-based analysis of five anterior and five posterior matched lobes to build the first lobe-specific pituitary proteome map, which documented 4090 proteins with isoforms, mostly mapped into chromosomes 1, 2, and 11. About 1446 differentially expressed significant proteins were identified, which were studied for lobe specificity, biological pathway enrichment, protein-protein interaction, regions specific to comparison of human brain and other neuroendocrine glands from Human Protein Atlas to identify pituitary-enriched proteins. Hormones specific to each lobe were also identified and validated with parallel reaction monitoring-based target verification. The study identified and validated hormones, growth hormone and thyroid-stimulating hormone subunit beta, exclusively to the anterior lobe whereas oxytocin-neurophysin 1 and arginine vasopressin to the posterior lobe. The study also identified proteins POU1F1 (pituitary-specific positive transcription factor 1), POMC (pro-opiomelanocortin), PCOLCE2 (procollagen C-endopeptidase enhancer 2), and NPTX2 (neuronal pentraxin-2) as pituitary-enriched proteins and was validated for their lobe specificity using parallel reaction monitoring. In addition, three uPE1 proteins, namely THEM6 (mesenchymal stem cell protein DSCD75), FSD1L (coiled-coil domain-containing protein 10), and METTL26 (methyltransferase-like 26), were identified using the NeXtProt database, and depicted tumor markers S100 proteins having high expression in the posterior lobe. In summary, the study documents the first matched anterior and posterior pituitary proteome map acting as a reference control for a better understanding of functional and nonfunctional pituitary adenomas and extrapolating the aim of the Human Proteome Project towards the investigation of the proteome of life.

Dataset Information

Proteomic Analysis of the Human Anterior Pituitary Gland.

Publications

Proteomic Analysis of the Human Anterior Pituitary Gland.

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