Unknown

Dataset Information

0

GATA6 suppresses migration and metastasis by regulating the miR-520b/CREB1 axis in gastric cancer.


ABSTRACT: Transcription factors (TFs) and microRNAs (miRNAs) are tightly linked to each other in tumor development and progression, but their interactions in gastric cancer (GC) metastasis remain elusive. Here we report a novel suppressive role of GATA6 in inhibiting GC metastasis by transactivating miR-520b. We found that GATA6 expression was significantly downregulated in metastatic GC cells and tissues and that its downregulation was correlated with a poor GC prognosis. Overexpression of GATA6 suppressed GC cell migration, invasion and metastasis both in vitro and in vivo. Luciferase reporter assays and chromatin immunoprecipitation assays demonstrated that miR-520b is a direct transcriptional target of GATA6. Moreover, miR-520b expression was positively correlated with GATA6 expression in GC tissues, and ectopic expression of miR-520b inhibited the migration and invasion of GC cells. Furthermore, cAMP responsive element binding protein 1 (CREB1) was identified as a direct and functional target of miR-520b, and GATA6 could suppress GC cell migration and metastasis via miR-520b-mediated repression of CREB1. Downregulation of GATA6 and miR-520b may partly account for the overexpression of CREB1 in GC. In conclusion, our results provide novel insight into the TF-miRNA regulatory network involved in GC metastasis. Targeting the GATA6/miR-520b/CREB1 axis may be an effective approach for GC treatment.

SUBMITTER: Liu H 

PROVIDER: S-EPMC6426848 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature

altmetric image

Publications

GATA6 suppresses migration and metastasis by regulating the miR-520b/CREB1 axis in gastric cancer.

Liu Hao H   Du Feng F   Sun Lina L   Wu Qingfeng Q   Wu Jian J   Tong Mingfu M   Wang Xin X   Wang Qi Q   Cao Tianyu T   Gao Xiaoliang X   Cao Jiayi J   Wu Nan N   Nie Yongzhan Y   Fan Daiming D   Lu Yuanyuan Y   Zhao Xiaodi X  

Cell death & disease 20190115 2


Transcription factors (TFs) and microRNAs (miRNAs) are tightly linked to each other in tumor development and progression, but their interactions in gastric cancer (GC) metastasis remain elusive. Here we report a novel suppressive role of GATA6 in inhibiting GC metastasis by transactivating miR-520b. We found that GATA6 expression was significantly downregulated in metastatic GC cells and tissues and that its downregulation was correlated with a poor GC prognosis. Overexpression of GATA6 suppress  ...[more]

Similar Datasets

| S-EPMC10238141 | biostudies-literature
| S-EPMC8018440 | biostudies-literature
| S-EPMC11402943 | biostudies-literature
| S-EPMC7597012 | biostudies-literature
| S-EPMC7816457 | biostudies-literature
| S-EPMC5561618 | biostudies-literature
| S-EPMC7567569 | biostudies-literature
| S-EPMC6945592 | biostudies-literature
| S-EPMC8973856 | biostudies-literature
| S-EPMC8312431 | biostudies-literature