Unknown

Dataset Information

0

Specification and Diversification of Pericytes and Smooth Muscle Cells from Mesenchymoangioblasts.

ABSTRACT: Elucidating the pathways that lead to vasculogenic cells, and being able to identify their progenitors and lineage-restricted cells, is critical to the establishment of human pluripotent stem cell (hPSC) models for vascular diseases and development of vascular therapies. Here, we find that mesoderm-derived pericytes (PCs) and smooth muscle cells (SMCs) originate from a clonal mesenchymal progenitor mesenchymoangioblast (MB). In clonogenic cultures, MBs differentiate into primitive PDGFR?+CD271+CD73- mesenchymal progenitors, which give rise to proliferative PCs, SMCs, and mesenchymal stem/stromal cells. MB-derived PCs can be further specified to CD274+ capillary and DLK1+ arteriolar PCs with a proinflammatory and contractile phenotype, respectively. SMC maturation was induced using a MEK inhibitor. Establishing the vasculogenic lineage tree, along with identification of stage- and lineage-specific markers, provides a platform for interrogating the molecular mechanisms that regulate vasculogenic cell specification and diversification and manufacturing well-defined mural cell populations for vascular engineering and cellular therapies from hPSCs.

SUBMITTER: Kumar A 

PROVIDER: S-EPMC6428685 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Specification and Diversification of Pericytes and Smooth Muscle Cells from Mesenchymoangioblasts.

Kumar Akhilesh A   D'Souza Saritha Sandra SS   Moskvin Oleg V OV   Toh Huishi H   Wang Bowen B   Zhang Jue J   Swanson Scott S   Guo Lian-Wang LW   Thomson James A JA   Slukvin Igor I II  

Cell reports 20170501 9

Elucidating the pathways that lead to vasculogenic cells, and being able to identify their progenitors and lineage-restricted cells, is critical to the establishment of human pluripotent stem cell (hPSC) models for vascular diseases and development of vascular therapies. Here, we find that mesoderm-derived pericytes (PCs) and smooth muscle cells (SMCs) originate from a clonal mesenchymal progenitor mesenchymoangioblast (MB). In clonogenic cultures, MBs differentiate into primitive PDGFRβ<sup>+</  ...[more]

Similar Datasets

Transcriptomics Specification and Diversification of Pericytes and Smooth Muscle Cells from Mesenchymoangioblasts
2017-05-30 | GSE75990 | GEO
Unknown Endothelial cells are progenitors of cardiac pericytes and vascular smooth muscle cells.
| S-EPMC4990645 | biostudies-literature
Unknown Inhibitory effect of caveolin-1 in vascular endothelial cells, pericytes and smooth muscle cells.
| S-EPMC5652695 | biostudies-literature
Unknown Tissue-Specific Cultured Human Pericytes: Perivascular Cells from Smooth Muscle Tissue Have Restricted Mesodermal Differentiation Ability.
| S-EPMC4854214 | biostudies-literature
Unknown Proteomic profiling of concurrently isolated primary microvascular endothelial cells, pericytes, and vascular smooth muscle cells from adult mouse heart.
| S-EPMC9132906 | biostudies-literature
Unknown Isolation and differential transcriptome of vascular smooth muscle cells and mid-capillary pericytes from the rat brain.
| S-EPMC6095852 | biostudies-literature
Unknown Tra2beta as a novel mediator of vascular smooth muscle diversification.
| S-EPMC2737447 | biostudies-literature
Unknown Vascular wall-resident CD44+ multipotent stem cells give rise to pericytes and smooth muscle cells and contribute to new vessel maturation.
| S-EPMC3102739 | biostudies-literature
Unknown An Engineered Adeno-Associated Virus Capsid Mediates Efficient Transduction of Pericytes and Smooth Muscle Cells of the Brain Vasculature.
| S-EPMC10457656 | biostudies-literature
Unknown Human primary endothelial cells are impaired in nucleotide excision repair and sensitive to benzo[a]pyrene compared with smooth muscle cells and pericytes.
| S-EPMC6760230 | biostudies-literature