Low Expression of IL-10 in Circulating Bregs and Inverted IL-10/TNF-? Ratio in Tears of Patients with Perennial Allergic Conjunctivitis: A Preliminary Study.

ABSTRACT: Allergic conjunctivitis (AC) is one of the most common ophthalmological disorders seen in clinical practice. Growing evidence from recent years suggests that a subset of IL-10-expressing B cells is involved in inflammatory allergic diseases. In this study, we aimed to evaluate the potential involvement of blood Bregs cells in perennial allergic conjunctivitis (PAC), and interleukins (IL)-1?, IL-6, IL-8, IL-10, and IL-12, and tumor necrosis factor (TNF)-?, were measured in tear samples and compared with healthy controls (HC) using flow cytometry. Non-significant differences in CD19?IL-10? cell frequency between PAC patients and healthy controls (HC) were observed. Nevertheless, when we analyzed the mean fluorescence intensity (MFI) of IL-10 on CD19?CD38Lo/Med/Hi-gated cells, we observed a significant decrease in MFI in all Bregs subsets in PAC patients. Additionally, tear cytokines showed 2.8 times lower levels of IL-10 than TNF-? in PAC patients when compared to HC. Our findings demonstrate an immunological dysregulation in patients with allergic conjunctivitis, characterized by the low expression of IL-10 in circulating CD19?CD38? Bregs subsets and an inverted tear IL-10/TNF-? ratio, promoting a local pro-inflammatory microenvironment. These findings highlight the novel pathologic changes involved in ocular allergic diseases. Understanding systemic and local mechanisms will aid the design of immunomodulating therapeutics at different levels.

SUBMITTER: Salazar A 

PROVIDER: S-EPMC6429471 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature