Dataset Information

Association of neutrophil-to-lymphocyte ratio and natural killer cell activity revealed by measurement of interferon-gamma levels in a healthy population.

ABSTRACT:

Background

While a method of assaying natural killer (NK) cell activity by measuring the amount of interferon (IFN)-? released from NK cells has been proposed, no data are available about the factors that influence IFN-? levels related to NK cell activity. NLR has recently been reported to be a predictor of several diseases. In the present study, we investigated the pre-analytical variables for NK cell activity using measurements of IFN-? and the relationship between NLR and NK cell activity.

Methods

The NK cell activity was assessed with the measurement of IFN-? after stimulation with an NK cell-specific stimulant (NK Vue^™ , ATgen, Sungnam, Korea). One hundred and six adult volunteers were recruited and analysis of their complete blood count data and serum C-reactive protein was done. Blood sample from 59 of the participants was also used for analysis of lymphocyte subpopulations.

Result

Natural killer cell activity varied widely (range, 44.2-1775.6 pg/mL). NK cell activity was higher in females than in males (P = 0.014). NK cell activity decreased with increasing NLR (P = 0.004, r = -0.32) but NK cell activity showed no significant association with NK cell count or other lymphocyte subpopulations. NK cell activity levels according to CRP quartile were significantly different (P = 0.025).

Conclusion

We have observed that NK cell activity when assessed by IFN-? level measurement was negatively correlating with NLR. This result can be helpful in interpreting or predicting NK cell activity in the clinical environment.

SUBMITTER: Kim BR

PROVIDER: S-EPMC6430355 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature

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Publications

Association of neutrophil-to-lymphocyte ratio and natural killer cell activity revealed by measurement of interferon-gamma levels in a healthy population.

Kim Bo-Ram BR Chun Sejong S Cho Duck D Kim Kyeong-Hee KH

Journal of clinical laboratory analysis 20180813 1

<h4>Background</h4>While a method of assaying natural killer (NK) cell activity by measuring the amount of interferon (IFN)-γ released from NK cells has been proposed, no data are available about the factors that influence IFN-γ levels related to NK cell activity. NLR has recently been reported to be a predictor of several diseases. In the present study, we investigated the pre-analytical variables for NK cell activity using measurements of IFN-γ and the relationship between NLR and NK cell acti ...[more]

PMID: 30105845

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Project description:BackgroundThe preoperative peripheral blood neutrophil-lymphocyte ratio (NLR), platelet-lymphocyte ratio (PLR) and monocyte-lymphocyte ratio (MLR) have been reported to be associated with the prognosis of various cancers but are always discussed separately. The aim of this study is to bring the combination of NLR, PLR and MLR into the prognostic assessment system of endometrial cancer (EC) and establish a nomogram to provide an objective prediction model for clinical decisions.MethodsA total of 1111 patients with EC who had accepted surgical treatment during 2013-2017 were involved in the analysis. Their NLR, PLR, and MLR levels were obtained from a routine blood examination within 2 weeks before operation. Receiver operating characteristic curve (ROC) analysis was performed to determine optimal cutoffs. Chi-square tests analysed the associations of the ratios with other clinicopathological variables. The prognostic value was indicated by overall survival (OS) via Cox proportional hazards models and Kaplan-Meier analysis. R software was used to establish the nomogram based on the combination of NLR, PLR, MLR and other clinicopathological factors.ResultsThe median follow-up period was 40 months, and the median age was 56. The enrolled patients were stratified by cutoffs of 2.14 for NLR, 131.82 for PLR and 0.22 for MLR. Multivariate analyses demonstrated that high NLR over 2.14 (HR = 2.71, 95%CI = 1.83-4.02, P<0.001), high PLR over 131.82 (HR = 2.75, 95%CI = 1.90-3.97, P<0.001), and high MLR over 0.22 (HR = 1.72, 95%CI = 1.20-2.45, P = 0.003) were significantly associated with worse OS. The combined indicator, high NLR + high PLR + high MLR (HR = 4.34, 95%CI = 2.54-7.42, P<0.001), showed the highest prognostic value. The Harrell's concordance index of the nomogram was 0.847 (95% CI = 0.804-0.890), showing good discrimination and calibration of this model.ConclusionThe combination of NLR, PLR, and MLR is a superior prognostic factor of EC. The nomogram involving the combination of NLR, PLR, MLR and other clinicopathological factors is recommended to predict OS for EC patients clinically.

Project description:BackgroundResearch on neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), and monocyte-to-lymphocyte ratio (MLR) in depression is still emerging and has increased 3-fold since the first meta-analysis. An updated meta-analysis with sufficient studies can provide more evidence for a potential relationship between NLR, PLR, MLR, and depression.MethodsWe identified 18 studies from the PubMed, EMBASE, Cochrane library, and Web of Science databases. Meta-analyses were performed to generate pooled standardized mean differences (SMDs) and 95% confidence intervals (CIs) between patients with depression and controls. Sensitivity analysis, subgroup analysis, meta-regression, and publication bias were conducted.ResultsA total of 18 studies including 2,264 depressed patients and 2,415 controls were included. Depressed patients had significantly higher NLR and PLR compared with controls (SMD = 0.33, 95% CI: 0.15-0.52, p < 0.001 and SMD = 0.24, 95% CI: 0.02-0.46, p < 0.05, respectively). MLR was slightly higher in depressed individuals compared to controls (SMD = 0.15, 95% CI: -0.26 to 0.55, p > 0.05), despite the absence of significance. Sensitivity analysis removing one study responsible for heterogeneity showed a higher and significant effect (SMD = 0.32, 95% CI: 0.20-0.44) of MLR. Three subgroup analyses of NLR, PLR, MLR, and depression revealed obvious differences in the inflammatory ratios between depressed patients and controls in China and the matched age and gender subgroup. Individuals with post-stroke depression (PSD) had higher NLR and MLR values as compared to non-PSD patients (SMD = 0.51, 95% CI: 0.36-0.67, p < 0.001 and SMD = 0.46, 95% CI: 0.12-0.79, p < 0.01, respectively). Meta-regression analyses showed that male proportion in the case group influenced the heterogeneity among studies that measured NLR values (p < 0.05).ConclusionsHigher inflammatory ratios, especially NLR, were significantly associated with an increased risk of depression. In the subgroup of China and matched age and gender, NLR, PLR, and MLR were all elevated in depressed patients vs. controls. Individuals with PSD had higher NLR and MLR values as compared to non-PSD patients. Gender differences may have an effect on NLR values in patients with depression.

Dataset Information

Association of neutrophil-to-lymphocyte ratio and natural killer cell activity revealed by measurement of interferon-gamma levels in a healthy population.

Background

Methods

Result

Conclusion

Publications

Association of neutrophil-to-lymphocyte ratio and natural killer cell activity revealed by measurement of interferon-gamma levels in a healthy population.

Similar Datasets

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