Ontology highlight
ABSTRACT:
SUBMITTER: Lee WD
PROVIDER: S-EPMC6430770 | biostudies-literature | 2019 Mar
REPOSITORIES: biostudies-literature
Lee Won Dong WD Mukha Dzmitry D Aizenshtein Elina E Shlomi Tomer T
Nature communications 20190322 1
The inability to inspect metabolic activities within subcellular compartments has been a major barrier to our understanding of eukaryotic cell metabolism. Here, we describe a spatial-fluxomics approach for inferring metabolic fluxes in mitochondria and cytosol under physiological conditions, combining isotope tracing, rapid subcellular fractionation, LC-MS-based metabolomics, computational deconvolution, and metabolic network modeling. Applied to study reductive glutamine metabolism in cancer ce ...[more]