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Nanoclays for Lipase Immobilization: Biocatalyst Characterization and Activity in Polyester Synthesis.

ABSTRACT: The immobilization of Candida antarctica lipase B (CALB) was performed by physical adsorption on both neat and organo-modified forms of sepiolite and montmorillonite. The influence of different parameters, e.g., solvent, enzyme loading, cross-linking, and type of clay support, on immobilization efficiency and catalyst hydrolytic activity has been investigated. The highest hydrolytic activities were obtained for CALB immobilized on organo-modified clay minerals, highlighting the beneficial effect of organo-modification. The esterification activity of these CALB/organoclay catalysts was also tested in the ring-opening polymerization of ?-caprolactone. The polymerization kinetics observed for clay-immobilized catalysts confirmed that CALB adsorbed on organo-modified montmorillonite (CALB/MMTMOD) was the highest-performing catalytic system.

SUBMITTER: Ozturk H

PROVIDER: S-EPMC6432194 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature

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Nanoclays for Lipase Immobilization: Biocatalyst Characterization and Activity in Polyester Synthesis.

Öztürk Hale H Pollet Eric E Phalip Vincent V Güvenilir Yüksel Y Avérous Luc L

Polymers 20161201 12

The immobilization of <i>Candida antarctica</i> lipase B (CALB) was performed by physical adsorption on both neat and organo-modified forms of sepiolite and montmorillonite. The influence of different parameters, e.g., solvent, enzyme loading, cross-linking, and type of clay support, on immobilization efficiency and catalyst hydrolytic activity has been investigated. The highest hydrolytic activities were obtained for CALB immobilized on organo-modified clay minerals, highlighting the beneficial ...[more]

PMID: 30974694

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