PPAR? regulates meibocyte differentiation and lipid synthesis of cultured human meibomian gland epithelial cells (hMGEC).
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ABSTRACT:
SUBMITTER: Kim SW
PROVIDER: S-EPMC6434942 | biostudies-literature | 2018 Oct
REPOSITORIES: biostudies-literature
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PPARγ regulates meibocyte differentiation and lipid synthesis of cultured human meibomian gland epithelial cells (hMGEC).
Kim Sun Woong SW Xie Yilu Y Nguyen Paul Q PQ Bui Vickie T VT Huynh Kelly K Kang Jonathan S JS Brown Donald J DJ Jester James V JV
The ocular surface 20180707 4
<h4>Purpose</h4>To evaluate the role of PPARγ in regulating meibocyte differentiation and lipid synthesis in a human meibomian gland epithelial cell line (hMGEC).<h4>Methods</h4>HMGEC were exposed to the PPARγ agonist, Rosiglitazone, from 10-50 μM. Cultures were also exposed to specific PPARγ antagonist, T0070907, to block PPARγ receptor signaling. Cells were then stained with Ki-67 and LipidTox to determine the effects on cell cycling and lipid synthesis, respectively. Expression of meibocyte d ...[more]
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