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Mitochondrial function in immature bovine oocytes is improved by an increase of cellular cyclic AMP.


ABSTRACT: Although in vitro maturation (IVM) of oocytes is important for assisted reproduction, the rate of development of embryos from IVM oocytes is lower than from their in vivo counterparts. It has been shown that an artificial increase of intracellular cAMP before culture significantly improves oocyte developmental competence in cattle and mice. Here, we revealed that forskolin and 3-isobutyl-1-methylxanthine treatment of prophase-stage oocytes induced the expression of genes required for glycolysis, fatty acid degradation, and the mitochondrial electron transport system and improved mitochondrial functions and ATP levels in oocytes without involving nuclear maturation. We propose the existence of a comprehensive energy-supply system in oocytes under follicle-stimulating hormone stimulation as a potential explanation of how oocytes acquire developmental competence.

SUBMITTER: Hashimoto S 

PROVIDER: S-EPMC6435665 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature

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Mitochondrial function in immature bovine oocytes is improved by an increase of cellular cyclic AMP.

Hashimoto Shu S   Yamanaka Masaya M   Yamochi Takayuki T   Iwata Hisataka H   Kawahara-Miki Ryouka R   Inoue Masayasu M   Morimoto Yoshiharu Y  

Scientific reports 20190326 1


Although in vitro maturation (IVM) of oocytes is important for assisted reproduction, the rate of development of embryos from IVM oocytes is lower than from their in vivo counterparts. It has been shown that an artificial increase of intracellular cAMP before culture significantly improves oocyte developmental competence in cattle and mice. Here, we revealed that forskolin and 3-isobutyl-1-methylxanthine treatment of prophase-stage oocytes induced the expression of genes required for glycolysis,  ...[more]

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