Highly Mutable Linker Regions Regulate HIV-1 Rev Function and Stability.

ABSTRACT: HIV-1 Rev is an essential viral regulatory protein that facilitates the nuclear export of intron-containing viral mRNAs. It is organized into structured, functionally well-characterized motifs joined by less understood linker regions. Our recent competitive deep mutational scanning study confirmed many known constraints in Rev's established motifs, but also identified positions of mutational plasticity, most notably in surrounding linker regions. Here, we probe the mutational limits of these linkers by testing the activities of multiple truncation and mass substitution mutations. We find that these regions possess previously unknown structural, functional or regulatory roles, not apparent from systematic point mutational approaches. Specifically, the N- and C-termini of Rev contribute to p

SUBMITTER: Jayaraman B 

PROVIDER: S-EPMC6435700 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature