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Developing a blood-based gene mutation assay as a novel biomarker for oesophageal adenocarcinoma.

ABSTRACT: The Phosphatidylinositol glycan class A (PIG-A) gene mutation assay phenotypically measures erythrocyte mutations, assessed here for their correlation to neoplastic progression in the gastro-oesophageal reflux disease (GORD)-Barrett's metaplasia (BM)-oesophageal adenocarcinoma (OAC) model. Endoscopy patients underwent venipuncture and erythrocytes fluorescently stained for glycosyl phosphatidylinositol (GPI)-anchored proteins; CD55 and CD59. Using flow cytometry, GPI-anchor negative erythrocytes (mutants) were scored and compared amongst groups. The study enlisted 200 patients and 137 healthy volunteers. OAC patients had a three-fold increase in erythrocyte mutant frequency (EMF) compared to GORD patients (p?

SUBMITTER: Haboubi HN

PROVIDER: S-EPMC6435702 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature

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Developing a blood-based gene mutation assay as a novel biomarker for oesophageal adenocarcinoma.

Haboubi Hasan N HN Lawrence Rachel L RL Rees Benjamin B Williams Lisa L Manson James M JM Al-Mossawi Neam N Bodger Owen O Griffiths Paul P Thornton Catherine C Jenkins Gareth J GJ

Scientific reports 20190326 1

The Phosphatidylinositol glycan class A (PIG-A) gene mutation assay phenotypically measures erythrocyte mutations, assessed here for their correlation to neoplastic progression in the gastro-oesophageal reflux disease (GORD)-Barrett's metaplasia (BM)-oesophageal adenocarcinoma (OAC) model. Endoscopy patients underwent venipuncture and erythrocytes fluorescently stained for glycosyl phosphatidylinositol (GPI)-anchored proteins; CD55 and CD59. Using flow cytometry, GPI-anchor negative erythrocytes ...[more]

PMID: 30914682

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