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ParAlleL: A Novel Population-Based Approach to Biological Logic Gates.

ABSTRACT: In vivo logic gates have proven difficult to combine into larger devices. Our cell-based logic system, ParAlleL, decomposes a large circuit into a collection of small subcircuits working in parallel, each subcircuit responding to a different combination of inputs. A final global output is then generated by a combination of the responses. Using ParAlleL, for the first time a completely functional 3-bit full adder and full subtractor were generated using Escherichia coli cells, as well as a calculator-style display that shows a numeric result, from 0 to 7, when the proper 3 bit binary inputs are introduced into the system. ParAlleL demonstrates the use of a parallel approach for the design of cell-based logic gates that facilitates the generation and analysis of complex processes, without the need for complex genetic engineering.

SUBMITTER: Millacura FA

PROVIDER: S-EPMC6437039 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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ParAlleL: A Novel Population-Based Approach to Biological Logic Gates.

Millacura Felipe A FA Largey Brendan B French Christopher E CE

Frontiers in bioengineering and biotechnology 20190321

<i>In vivo</i> logic gates have proven difficult to combine into larger devices. Our cell-based logic system, ParAlleL, decomposes a large circuit into a collection of small subcircuits working in parallel, each subcircuit responding to a different combination of inputs. A final global output is then generated by a combination of the responses. Using ParAlleL, for the first time a completely functional 3-bit full adder and full subtractor were generated using <i>Escherichia coli</i> cells, as we ...[more]

PMID: 30949475

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