Ontology highlight
ABSTRACT:
SUBMITTER: Landry MP
PROVIDER: S-EPMC6438169 | biostudies-literature | 2017 May
REPOSITORIES: biostudies-literature
Landry Markita Patricia MP Ando Hiroki H Chen Allen Y AY Cao Jicong J Kottadiel Vishal Isaac VI Chio Linda L Yang Darwin D Dong Juyao J Lu Timothy K TK Strano Michael S MS
Nature nanotechnology 20170123 4
A distinct advantage of nanosensor arrays is their ability to achieve ultralow detection limits in solution by proximity placement to an analyte. Here, we demonstrate label-free detection of individual proteins from Escherichia coli (bacteria) and Pichia pastoris (yeast) immobilized in a microfluidic chamber, measuring protein efflux from single organisms in real time. The array is fabricated using non-covalent conjugation of an aptamer-anchor polynucleotide sequence to near-infrared emissive si ...[more]