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IntensityCheck - The light measuring app for microscope performance checks and consistent fluorescence imaging.

ABSTRACT: Quantitative fluorescence imaging is an essential tool in biomedical research. It requires consistent and repeatable conditions such as constant sample illumination. Even on a confocal microscope this can usually only be achieved by using an external laser power meter. By combining low-cost wireless Arduino based light sensors with an easy to use Android smartphone app we provide microscope users with a simple but powerful tool to maintain sample illumination for quantitative imaging, for tracking the intensity, stability and alignment of the light sources and for comparing microscope performance.

SUBMITTER: Dormann D

PROVIDER: S-EPMC6438524 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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IntensityCheck - The light measuring app for microscope performance checks and consistent fluorescence imaging.

Dormann Dirk D

PloS one 20190328 3

Quantitative fluorescence imaging is an essential tool in biomedical research. It requires consistent and repeatable conditions such as constant sample illumination. Even on a confocal microscope this can usually only be achieved by using an external laser power meter. By combining low-cost wireless Arduino based light sensors with an easy to use Android smartphone app we provide microscope users with a simple but powerful tool to maintain sample illumination for quantitative imaging, for tracki ...[more]

PMID: 30921439

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