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Intramolecular domain dynamics regulate synaptic MAGUK protein interactions.


ABSTRACT: PSD-95 MAGUK family scaffold proteins are multi-domain organisers of synaptic transmission that contain three PDZ domains followed by an SH3-GK domain tandem. This domain architecture allows coordinated assembly of protein complexes composed of neurotransmitter receptors, synaptic adhesion molecules and downstream signalling effectors. Here we show that binding of monomeric CRIPT-derived PDZ3 ligands to the third PDZ domain of PSD-95 induces functional changes in the intramolecular SH3-GK domain assembly that influence subsequent homotypic and heterotypic complex formation. We identify PSD-95 interactors that differentially bind to the SH3-GK domain tandem depending on its conformational state. Among these interactors, we further establish the heterotrimeric G protein subunit Gnb5 as a PSD-95 complex partner at dendritic spines of rat hippocampal neurons. The PSD-95 GK domain binds to Gnb5, and this interaction is triggered by CRIPT-derived PDZ3 ligands binding to the third PDZ domain of PSD-95, unraveling a hierarchical binding mechanism of PSD-95 complex formation.

SUBMITTER: Rademacher N 

PROVIDER: S-EPMC6438691 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature

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Intramolecular domain dynamics regulate synaptic MAGUK protein interactions.

Rademacher Nils N   Kuropka Benno B   Kunde Stella-Amrei SA   Wahl Markus C MC   Freund Christian C   Shoichet Sarah A SA  

eLife 20190313


PSD-95 MAGUK family scaffold proteins are multi-domain organisers of synaptic transmission that contain three PDZ domains followed by an SH3-GK domain tandem. This domain architecture allows coordinated assembly of protein complexes composed of neurotransmitter receptors, synaptic adhesion molecules and downstream signalling effectors. Here we show that binding of monomeric CRIPT-derived PDZ<sub>3</sub> ligands to the third PDZ domain of PSD-95 induces functional changes in the intramolecular SH  ...[more]

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