Impact of the secretome of activated pancreatic stellate cells on growth and differentiation of pancreatic tumour cells.

ABSTRACT: Pancreatic ductal adenocarcinoma (PDAC) exists in a complex desmoplastic microenvironment. As part of it, pancreatic stellate cells (PSCs) provide a fibrotic niche, stimulated by a dynamic communication between activated PSCs and tumour cells. Investigating how PSCs contribute to tumour development and for identifying proteins that the cells secrete during cancer progression, we studied by means of complex antibody microarrays the secretome of activated PSCs. A large number of secretome proteins were associated with cancer-related functions, such as cell apoptosis, cellular growth, proliferation and metastasis. Their effect on tumour cells could be confirmed by growing tumour cells in medium conditioned with activated PSC secretome. Analyses of the tumour cells' proteome and mRNA revealed

SUBMITTER: Marzoq AJ 

PROVIDER: S-EPMC6438963 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature