Using Microfluidics and Imaging SAMDI-MS To Characterize Reaction Kinetics.

ABSTRACT: Microfluidic platforms have enabled the simplification of biochemical assays with a significant reduction in the use of reagents, yet the current methods available for analyzing reaction products can limit applications of these approaches. This paper demonstrates a simple microfluidic device that incorporates a functionalized self-assembled monolayer to measure the rate constant for a chemical reaction. The device mixes the reactants and allows them to selectively immobilize to the monolayer at the base of a microfluidic channel in a time-dependent manner as they flow down the channel. Imaging self-assembled monolayers for matrix-assisted laser desorption/ionization mass spectrometry (iSAMDI-MS) is used to acquire a quantitative image representing the time-resolved progress of the reaction

SUBMITTER: Grant J 

PROVIDER: S-EPMC6439460 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature