Dataset Information

Biodegradation of thiocyanate by a native groundwater microbial consortium.

ABSTRACT: Gold ore processing typically generates large amounts of thiocyanate (SCN-)-contaminated effluent. When this effluent is stored in unlined tailings dams, contamination of the underlying aquifer can occur. The potential for bioremediation of SCN--contaminated groundwater, either in situ or ex situ, remains largely unexplored. This study aimed to enrich and characterise SCN--degrading microorganisms from mining-contaminated groundwater under a range of culturing conditions. Mildly acidic and suboxic groundwater, containing ?135 mg L-1 SCN-, was collected from an aquifer below an unlined tailings dam. An SCN--degrading consortium was enriched from contaminated groundwater using combinatory amendments of air, glucose and phosphate. Biodegradation occurred in all oxic cultures, except with the sole addition of glucose, but was inhibited by NH4 + and did not occur under anoxic conditions. The SCN--degrading consortium was characterised using 16S and 18S rRNA gene sequencing, identifying a variety of heterotrophic taxa in addition to sulphur-oxidising bacteria. Interestingly, few recognised SCN--degrading taxa were identified in significant abundance. These results provide both proof-of-concept and the required conditions for biostimulation of SCN- degradation in groundwater by native aquifer microorganisms.

SUBMITTER: Spurr LP

PROVIDER: S-EPMC6440457 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Biodegradation of thiocyanate by a native groundwater microbial consortium.

Spurr Liam P LP Watts Mathew P MP Gan Han M HM Moreau John W JW

PeerJ 20190326

Gold ore processing typically generates large amounts of thiocyanate (SCN-)-contaminated effluent. When this effluent is stored in unlined tailings dams, contamination of the underlying aquifer can occur. The potential for bioremediation of SCN--contaminated groundwater, either in situ or ex situ, remains largely unexplored. This study aimed to enrich and characterise SCN--degrading microorganisms from mining-contaminated groundwater under a range of culturing co ...[more]

PMID: 30941266

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Project description:Three kinds of alkaliphilic bacteria able to utilize thiocyanate (CNS-) at pH 10 were found in highly alkaline soda lake sediments and soda soils. The first group included obligate heterotrophs that utilized thiocyanate as a nitrogen source while growing at pH 10 with acetate as carbon and energy sources. Most of the heterotrophic strains were able to oxidize sulfide and thiosulfate to tetrathionate. The second group included obligately autotrophic sulfur-oxidizing alkaliphiles which utilized thiocyanate nitrogen during growth with thiosulfate as the energy source. Genetic analysis demonstrated that both the heterotrophic and autotrophic alkaliphiles that utilized thiocyanate as a nitrogen source were related to the previously described sulfur-oxidizing alkaliphiles belonging to the gamma subdivision of the division Proteobacteria (the Halomonas group for the heterotrophs and the genus Thioalkalivibrio for autotrophs). The third group included obligately autotrophic sulfur-oxidizing alkaliphilic bacteria able to utilize thiocyanate as a sole source of energy. These bacteria could be enriched on mineral medium with thiocyanate at pH 10. Growth with thiocyanate was usually much slower than growth with thiosulfate, although the biomass yield on thiocyanate was higher. Of the four strains isolated, the three vibrio-shaped strains were genetically closely related to the previously described sulfur-oxidizing alkaliphiles belonging to the genus Thioalkalivibrio. The rod-shaped isolate differed from the other isolates by its ability to accumulate large amounts of elemental sulfur inside its cells and by its ability to oxidize carbon disulfide. Despite its low DNA homology with and substantial phenotypic differences from the vibrio-shaped strains, this isolate also belonged to the genus Thioalkalivibrio according to a phylogenetic analysis. The heterotrophic and autotrophic alkaliphiles that grew with thiocyanate as an N source possessed a relatively high level of cyanase activity which converted cyanate (CNO-) to ammonia and CO2. On the other hand, cyanase activity either was absent or was present at very low levels in the autotrophic strains grown on thiocyanate as the sole energy and N source. As a result, large amounts of cyanate were found to accumulate in the media during utilization of thiocyanate at pH 10 in batch and thiocyanate-limited continuous cultures. This is a first direct proof of a "cyanate pathway" in pure cultures of thiocyanate-degrading bacteria. Since it is relatively stable under alkaline conditions, cyanate is likely to play a role as an N buffer that keeps the alkaliphilic bacteria safe from inhibition by free ammonia, which otherwise would reach toxic levels during dissimilatory degradation of thiocyanate.

Dataset Information

Biodegradation of thiocyanate by a native groundwater microbial consortium.

Publications

Biodegradation of thiocyanate by a native groundwater microbial consortium.

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