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How a circularized tmRNA moves through the ribosome.

ABSTRACT: During trans-translation, transfer-messenger RNA (tmRNA) and small protein B (SmpB) together rescue ribosomes stalled on a truncated mRNA and tag the nascent polypeptide for degradation. We used cryo-electron microscopy to determine the structures of three key states of the tmRNA-SmpB-ribosome complex during trans translation at resolutions of 3.7 to 4.4 angstroms. The results show how tmRNA and SmpB act specifically on stalled ribosomes and how the circularized complex moves through the ribosome, enabling translation to switch from the old defective message to the reading frame on tmRNA.

SUBMITTER: Rae CD

PROVIDER: S-EPMC6440651 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature

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How a circularized tmRNA moves through the ribosome.

Rae Christopher D CD Gordiyenko Yuliya Y Ramakrishnan V V

Science (New York, N.Y.) 20190201 6428

During trans-translation, transfer-messenger RNA (tmRNA) and small protein B (SmpB) together rescue ribosomes stalled on a truncated mRNA and tag the nascent polypeptide for degradation. We used cryo-electron microscopy to determine the structures of three key states of the tmRNA-SmpB-ribosome complex during trans translation at resolutions of 3.7 to 4.4 angstroms. The results show how tmRNA and SmpB act specifically on stalled ribosomes and how the circularized complex moves through the ribosom ...[more]

PMID: 30765567

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