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Computer aided protein engineering to enhance the thermo-stability of CXCR1- T4 lysozyme complex.


ABSTRACT: CXCR1, a member in G-protein coupled receptor (GPCR) family, binds to chemokine interleukin-8 (IL-8) specifically and transduces signals to mediate immune and inflammatory responses. Despite the importance of CXCR1, high-resolution structure determination is hindered by the challenges in crystallization. It has been shown that properly designed mutants with enhanced thermostability, together with fusion partner proteins, can be useful to form crystals for GPCR proteins. In this study, in silico protein design was carried out by using homology modeling and molecular dynamics simulations. To validate the computational modeling results, the thermostability of several mutants and the wild type were measured experimentally. Both computational results and experimental data suggest that the mutant L126W has a significant improvement in the thermostability. This study demonstrated that in silico design can guide protein engineering and potentially facilitate protein crystallography research.

SUBMITTER: Wang Y 

PROVIDER: S-EPMC6441008 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature

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Computer aided protein engineering to enhance the thermo-stability of CXCR1- T4 lysozyme complex.

Wang Yang Y   Park Jae-Hyun JH   Lupala Cecylia Severin CS   Yun Ji-Hye JH   Jin Zeyu Z   Huang Lanqing L   Li Xuanxuan X   Tang Leihan L   Lee Weontae W   Liu Haiguang H  

Scientific reports 20190329 1


CXCR1, a member in G-protein coupled receptor (GPCR) family, binds to chemokine interleukin-8 (IL-8) specifically and transduces signals to mediate immune and inflammatory responses. Despite the importance of CXCR1, high-resolution structure determination is hindered by the challenges in crystallization. It has been shown that properly designed mutants with enhanced thermostability, together with fusion partner proteins, can be useful to form crystals for GPCR proteins. In this study, in silico  ...[more]

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