Ontology highlight
ABSTRACT:
SUBMITTER: Malacaria E
PROVIDER: S-EPMC6441034 | biostudies-literature | 2019 Mar
REPOSITORIES: biostudies-literature
Malacaria Eva E Pugliese Giusj Monia GM Honda Masayoshi M Marabitti Veronica V Aiello Francesca Antonella FA Spies Maria M Franchitto Annapaola A Pichierri Pietro P
Nature communications 20190329 1
Stabilisation of stalled replication forks prevents excessive fork reversal and their pathological degradation, which can undermine genome integrity. Here we investigate a physiological role of RAD52 at stalled replication forks by using human cell models depleted of RAD52, a specific small-molecule inhibitor of the RAD52-ssDNA interaction, in vitro and single-molecule analyses. We demonstrate that RAD52 prevents excessive degradation of reversed replication forks by MRE11. Mechanistically, RAD5 ...[more]