Ontology highlight
ABSTRACT:
SUBMITTER: Dillard KE
PROVIDER: S-EPMC6441324 | biostudies-literature | 2018 Nov
REPOSITORIES: biostudies-literature
Dillard Kaylee E KE Brown Maxwell W MW Johnson Nicole V NV Xiao Yibei Y Dolan Adam A Hernandez Erik E Dahlhauser Samuel D SD Kim Yoori Y Myler Logan R LR Anslyn Eric V EV Ke Ailong A Finkelstein Ilya J IJ
Cell 20181018 4
CRISPR-Cas systems confer an adaptive immunity against viruses. Following viral injection, Cas1-Cas2 integrates segments of the viral genome (spacers) into the CRISPR locus. In type I CRISPR-Cas systems, efficient "primed" spacer acquisition and viral degradation (interference) require both the Cascade complex and the Cas3 helicase/nuclease. Here, we present single-molecule characterization of the Thermobifida fusca (Tfu) primed acquisition complex (PAC). We show that TfuCascade rapidly samples ...[more]