Dataset Information
In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain.
Ontology highlight
ABSTRACT:
SUBMITTER: Ouzounov DG
PROVIDER: S-EPMC6441362 | biostudies-literature | 2017 Apr
REPOSITORIES: biostudies-literature
Publications
In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain.
Nature methods 20170220 4
High-resolution optical imaging is critical to understanding brain function. We demonstrate that three-photon microscopy at 1,300-nm excitation enables functional imaging of GCaMP6s-labeled neurons beyond the depth limit of two-photon microscopy. We record spontaneous activity from up to 150 neurons in the hippocampal stratum pyramidale at ∼1-mm depth within an intact mouse brain. Our method creates opportunities for noninvasive recording of neuronal activity with high spatial and temporal resol ...[more]