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In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain.

ABSTRACT: High-resolution optical imaging is critical to understanding brain function. We demonstrate that three-photon microscopy at 1,300-nm excitation enables functional imaging of GCaMP6s-labeled neurons beyond the depth limit of two-photon microscopy. We record spontaneous activity from up to 150 neurons in the hippocampal stratum pyramidale at ?1-mm depth within an intact mouse brain. Our method creates opportunities for noninvasive recording of neuronal activity with high spatial and temporal resolution deep within scattering brain tissues.

SUBMITTER: Ouzounov DG

PROVIDER: S-EPMC6441362 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature

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In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain.

Ouzounov Dimitre G DG Wang Tianyu T Wang Mengran M Feng Danielle D DD Horton Nicholas G NG Cruz-Hernández Jean C JC Cheng Yu-Ting YT Reimer Jacob J Tolias Andreas S AS Nishimura Nozomi N Xu Chris C

Nature methods 20170220 4

High-resolution optical imaging is critical to understanding brain function. We demonstrate that three-photon microscopy at 1,300-nm excitation enables functional imaging of GCaMP6s-labeled neurons beyond the depth limit of two-photon microscopy. We record spontaneous activity from up to 150 neurons in the hippocampal stratum pyramidale at ∼1-mm depth within an intact mouse brain. Our method creates opportunities for noninvasive recording of neuronal activity with high spatial and temporal resol ...[more]

PMID: 28218900

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