Ontology highlight
ABSTRACT: Background
Glioma is the most lethal primary brain tumor, the survival rate still isn't improved in the past decades. It's essential to study the regulatory mechanism of glioma progression, hoping to find new therapy targets or methods. The family of tripartite motif (TRIM) containing proteins are E3 ubiquitination ligases, which play critical role in various tumor progression.Methods
Cell proliferation and invasion were analyzed by colony formation assay, soft agar growth assay, BrdU incorporation assay and transwell invasion assay. Luciferase reporter analysis was used to analyze NF-?B pathway activity.Results
We found TRIM31 was upregulated in glioma cells and tissues, its overexpression significantly promoted glioma cell proliferation and invasion, while its knockdown significantly inhibited glioma cell proliferation and invasion. Mechanism analysis found TRIM31 promoted NF-?B pathway activity and increased its targets expression. NF-?B inhibition reversed the phenotype caused by TRIM31, confirming TRIM31 promoted glioma progression through activating NF-?B pathway. Using clinical specimens found TRIM31 expression was positively correlative with NF-?B activity.Conclusion
This study found TRIM31 promoted glioma proliferation and invasion through activating NF-?B activity.
SUBMITTER: Zhou L
PROVIDER: S-EPMC6441556 | biostudies-literature | 2019
REPOSITORIES: biostudies-literature
Zhou Li L Deng Zhe-Zhi ZZ Li Hai-Yan HY Jiang Nan N Wei Zhi-Sheng ZS Hong Ming-Fan MF Chen Xiao-Dang XD Wang Ji-Hui JH Zhang Ming-Xing MX Shi Yi-Hua YH Lu Zheng-Qi ZQ Huang Xu-Ming XM
OncoTargets and therapy 20190327
<h4>Background</h4>Glioma is the most lethal primary brain tumor, the survival rate still isn't improved in the past decades. It's essential to study the regulatory mechanism of glioma progression, hoping to find new therapy targets or methods. The family of tripartite motif (TRIM) containing proteins are E3 ubiquitination ligases, which play critical role in various tumor progression.<h4>Methods</h4>Cell proliferation and invasion were analyzed by colony formation assay, soft agar growth assay, ...[more]