Dataset Information

Data on the enzymatic conversion of alkaline peroxide oxidative pretreated sugarcane bagasse for the production of fermentable sugars.

ABSTRACT: Central composite design (CCD) approach of the response surface methodology design of experiment was adopted to determine the production of fermentable sugars after enzymatic conversion of alkaline peroxide oxidative pretreated sugarcane bagasse lignocellulose. MINITAB 16 statistical software was used to design the experiments, evaluate and interpret data generated during the process. The effects of factors such as time, hydrogen peroxide concentration, and temperature on treated biomass for reducing sugars (RS) production were investigated. Operating pretreatment conditions (low-high design levels) were reaction time (6-10 h), hydrogen peroxide concentrations (1-3%v/v), and reaction temperature (60-90 °C). With the desirability of optimization of 1.000, optimal reducing sugar yield after enzymatic hydrolysis was validated to be at 100.2 °C, reaction time of 4.6 h, and hydrogen peroxide concentration of 0.3% with optimum RS yield of 153.74 mg equivalent glucose/g biomass.

SUBMITTER: Ayeni AO

PROVIDER: S-EPMC6441737 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Data on the enzymatic conversion of alkaline peroxide oxidative pretreated sugarcane bagasse for the production of fermentable sugars.

Ayeni Augustine O AO Oyekunle Daniel T DT Shodipe Oluwajimi C OC Folayan Johnson A JA

Data in brief 20190320

Central composite design (CCD) approach of the response surface methodology design of experiment was adopted to determine the production of fermentable sugars after enzymatic conversion of alkaline peroxide oxidative pretreated sugarcane bagasse lignocellulose. MINITAB 16 statistical software was used to design the experiments, evaluate and interpret data generated during the process. The effects of factors such as time, hydrogen peroxide concentration, and temperature on treated biomass for red ...[more]

PMID: 30976639

Similar Datasets

Project description:BackgroundLignocellulosic biomass has been commonly regarded as a potential feedstock for the production of biofuels and biochemicals. High sugar yields and the complete bioconversion of all the lignocellulosic sugars into valuable products are attractive for the utilization of lignocelluloses. It is essential to pretreat and hydrolyze lignocelluloses at high solids loadings during industrial processes, which is more economical and environmentally friendly as capital cost, energy consumption, and water usage can be reduced. However, oligosaccharides are inevitably released during the high solids loading enzymatic hydrolysis and they are more recalcitrant than monosaccharides for microorganisms.ResultsA fed-batch enzymatic hydrolysis of corn stover pretreated by the sodium hydroxide-methanol solution (SMs) at high solids loading was demonstrated to reach the high concentrations and yields of fermentable sugars. Glucose, xylose, cello-oligosaccharides, and xylo-oligosaccharides achieved 146.7 g/L, 58.7 g/L, 15.6 g/L, and 24.7 g/L, respectively, when the fed-batch hydrolysis was started at 12% (w/v) solids loading, and 7% fresh substrate and a standardized blend of cellulase, β-glucosidase, and hemicellulase were fed consecutively at 3, 6, 24, and 48 h to achieve a final solids loading of 40% (w/v). The total conversion of glucan and xylan reached 89.5% and 88.5%, respectively, when the oligosaccharides were taken into account. Then, a fed-batch culture on the hydrolysates was investigated for lipid production by Cutaneotrichosporon oleaginosum. Biomass, lipid content, and lipid yield were 50.7 g/L, 61.7%, and 0.18 g/g, respectively. The overall consumptions of cello-oligosaccharides and xylo-oligosaccharides reached 74.1% and 68.2%, respectively.ConclusionsHigh sugars concentrations and yields were achieved when the enzyme blend was supplemented simultaneously with the substrate at each time point of feeding during the fed-batch enzymatic hydrolysis. Oligosaccharides were co-utilized with monosaccharides during the fed-batch culture of C. oleaginosum. These results provide a promising strategy to hydrolyze alkaline organosolv-pretreated corn stover into fermentable sugars with high concentrations and yields for microbial lipid production.

Dataset Information

Data on the enzymatic conversion of alkaline peroxide oxidative pretreated sugarcane bagasse for the production of fermentable sugars.

Publications

Data on the enzymatic conversion of alkaline peroxide oxidative pretreated sugarcane bagasse for the production of fermentable sugars.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets