Dataset Information

Mo₂C-induced hydrogen production enhances microbial electrosynthesis of acetate from CO₂ reduction.

ABSTRACT:

Background

Microbial electrosynthesis (MES) is a biocathode-driven process, in which electroautotrophic microorganisms can directly uptake electrons or indirectly via H₂ from the cathode as energy sources and CO₂ as only carbon source to produce chemicals.

Results

This study demonstrates that a hydrogen evolution reaction (HER) catalyst can enhance MES performance. An active HER electrocatalyst molybdenum carbide (Mo₂C)-modified electrode was constructed for MES. The volumetric acetate production rate of MES with 12 mg cm^-2 Mo₂C was 0.19?±?0.02 g L^-1 day^-1, which was 2.1 times higher than that of the control. The final acetate concentration reached 5.72?±?0.6 g L^-1 within 30 days, and coulombic efficiencies of 64?±?0.7% were yielded. Furthermore, electrochemical study, scanning electron microscopy, and microbial community analyses suggested that Mo₂C can accelerate the release of hydrogen, promote the formation of biofilms and regulate the mixed microbial flora.

Conclusion

Coupling a HER catalyst to a cathode of MES system is a promising strategy for improving MES efficiency.

SUBMITTER: Tian S

PROVIDER: S-EPMC6442412 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Publications

Mo<sub>2</sub>C-induced hydrogen production enhances microbial electrosynthesis of acetate from CO<sub>2</sub> reduction.

Tian Shihao S Wang Haoqi H Dong Zhiwei Z Yang Yang Y Yuan Hao H Huang Qiong Q Song Tian-Shun TS Xie Jingjing J

Biotechnology for biofuels 20190401

<h4>Background</h4>Microbial electrosynthesis (MES) is a biocathode-driven process, in which electroautotrophic microorganisms can directly uptake electrons or indirectly via H<sub>2</sub> from the cathode as energy sources and CO<sub>2</sub> as only carbon source to produce chemicals.<h4>Results</h4>This study demonstrates that a hydrogen evolution reaction (HER) catalyst can enhance MES performance. An active HER electrocatalyst molybdenum carbide (Mo<sub>2</sub>C)-modified electrode was const ...[more]

PMID: 30976321

Similar Datasets

Project description:BackgroundEthyl acetate (C4H8O2) and hydrogen (H2) are industrially relevant compounds that preferably are produced via sustainable, non-petrochemical production processes. Both compounds are volatile and can be produced by Escherichia coli before. However, relatively low yields for hydrogen are obtained and a mix of by-products renders the sole production of hydrogen by micro-organisms unfeasible. High yields for ethyl acetate have been achieved, but accumulation of formate remained an undesired but inevitable obstacle. Coupling ethyl acetate production to the conversion of formate into H2 may offer an interesting solution to both drawbacks. Ethyl acetate production requires equimolar amounts of ethanol and acetyl-CoA, which enables a redox neutral fermentation, without the need for production of by-products, other than hydrogen and CO2.ResultsWe engineered Escherichia coli towards improved conversion of formate into H2 and CO2 by inactivating the formate hydrogen lyase repressor (hycA), both uptake hydrogenases (hyaAB, hybBC) and/or overexpressing the hydrogen formate lyase activator (fhlA), in an acetate kinase (ackA) and lactate dehydrogenase (ldhA)-deficient background strain. Initially 10 strains, with increasing number of modifications were evaluated in anaerobic serum bottles with respect to growth. Four reference strains ΔldhAΔackA, ΔldhAΔackA p3-fhlA, ΔldhAΔackAΔhycAΔhyaABΔhybBC and ΔldhAΔackAΔhycAΔhyaABΔhybBC p3-fhlA were further equipped with a plasmid carrying the heterologous ethanol acyltransferase (Eat1) from Wickerhamomyces anomalus and analyzed with respect to their ethyl acetate and hydrogen co-production capacity. Anaerobic co-production of hydrogen and ethyl acetate via Eat1 was achieved in 1.5-L pH-controlled bioreactors. The cultivation was performed at 30 °C in modified M9 medium with glucose as the sole carbon source. Anaerobic conditions and gas stripping were established by supplying N2 gas.ConclusionsWe showed that the engineered strains co-produced ethyl acetate and hydrogen to yields exceeding 70% of the pathway maximum for ethyl acetate and hydrogen, and propose in situ product removal via gas stripping as efficient technique to isolate the products of interest.

Dataset Information

Mo₂C-induced hydrogen production enhances microbial electrosynthesis of acetate from CO₂ reduction.

Background

Results

Conclusion

Publications

Mo<sub>2</sub>C-induced hydrogen production enhances microbial electrosynthesis of acetate from CO<sub>2</sub> reduction.

Similar Datasets

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Dataset Information

Mo2C-induced hydrogen production enhances microbial electrosynthesis of acetate from CO2 reduction.

Background

Results

Conclusion

Publications

Mo<sub>2</sub>C-induced hydrogen production enhances microbial electrosynthesis of acetate from CO<sub>2</sub> reduction.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Mo₂C-induced hydrogen production enhances microbial electrosynthesis of acetate from CO₂ reduction.