Ontology highlight
ABSTRACT:
SUBMITTER: Li Z
PROVIDER: S-EPMC6443403 | biostudies-literature | 2018 Sep
REPOSITORIES: biostudies-literature
Li Zhonghan Z Cheng Hanjun H Shao Shiqun S Lu Xiang X Mo Li L Tsang Jonathan J Zeng Pu P Guo Zhili Z Wang Siwen S Nathanson David A DA Heath James R JR Wei Wei W Xue Min M
Angewandte Chemie (International ed. in English) 20180806 36
An analytical method is described for profiling lactate production in single cells via the use of coupled enzyme reactions on surface-grafted resazurin molecules. The immobilization of the redox-labile probes was achieved through chemical modifications on resazurin, followed by bio-orthogonal click reactions. The lactate detection was demonstrated to be sensitive and specific. The method was incorporated into a single-cell barcode chip for simultaneous quantification of aerobic glycolysis activi ...[more]