Development and application of multiplex PCR method for simultaneous detection of seven viruses in ducks.

ABSTRACT:

Background

Major viruses, including duck-origin avian influenza virus, duck-origin Newcastle disease virus, novel duck parvovirus, duck hepatitis A virus, duck Tembusu virus, fowl adenovirus, and duck enteritis virus, pose great harm to ducks and cause enormous economic losses to duck industry. This study aims to establish a multiplex polymerase chain reaction (m-PCR) method for simultaneous detection of these seven viruses.

Results

Specific primers were designed and synthesized according to the conserved region of seven viral gene sequences. Then, seven recombinant plasmids, as the positive controls, were reconstructed in this study. Within the study, D-optimal design was adopted to optimize PCR parameters. The optimum parameters for m-PCR were annealing temperature at 57 °

SUBMITTER: Yao M 

PROVIDER: S-EPMC6444421 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature