Ontology highlight
ABSTRACT:
SUBMITTER: Knapp DJHF
PROVIDER: S-EPMC6445147 | biostudies-literature | 2019 Apr
REPOSITORIES: biostudies-literature
Knapp David J H F DJHF Michaels Yale S YS Jamilly Max M Ferry Quentin R V QRV Barbosa Hector H Milne Thomas A TA Fulga Tudor A TA
Nature communications 20190402 1
Spatial/temporal control of Cas9 guide RNA expression could considerably expand the utility of CRISPR-based technologies. Current approaches based on tRNA processing offer a promising strategy but suffer from high background. Here, to address this limitation, we present a screening platform which allows simultaneous measurements of the promoter strength, 5', and 3' processing efficiencies across a library of tRNA variants. This analysis reveals that the sequence determinants underlying these act ...[more]