Dataset Information

Activin promotes growth and antral cavity expansion in the dog ovarian follicle.

ABSTRACT: Understanding regulators of folliculogenesis remains limited in the domestic dog (Canis familiaris), which challenges our ability to develop in vitro follicle culture systems for canid genome rescue efforts. Here, we investigated the influence of activin on dog follicle development and survival, oocyte quality, and FSH receptor expression in culture. Preantral (150 - ?230??m diameter), early antral (231 - ?330??m), and antral (>330-550??m) stage follicles were encapsulated in a fibrin-alginate hydrogel with 0, 100, or 200?ng/ml rhActivin plus 0, 0.1, 1, or 10??g/ml FSH for 12 or 21?d of in vitro culture. All follicle groups increased in diameter (P?630??m) in early antral and antral cohorts. This complementary effect was not linked to changes in FSHR mRNA expression (P?>?0.05). Although not influencing (P?>?0.05) follicle survival or transzonal projection (TZP) density in shorter term 12?d culture, activin in the presence of 1?ng/ml FSH maintained TZP density from the 12-21?d interval. Activin also increased oocyte diameter and improved nuclear integrity compared to un-supplemented controls. These results indicate that activin acts synergistically with FSH to promote growth and antral cavity expansion of the dog follicle in vitro, information useful to formulating an effective culture microenvironment for this species.

SUBMITTER: Nagashima JB

PROVIDER: S-EPMC6445547 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

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Activin promotes growth and antral cavity expansion in the dog ovarian follicle.

Nagashima Jennifer B JB Wildt David E DE Travis Alexander J AJ Songsasen Nucharin N

Theriogenology 20190225

Understanding regulators of folliculogenesis remains limited in the domestic dog (Canis familiaris), which challenges our ability to develop in vitro follicle culture systems for canid genome rescue efforts. Here, we investigated the influence of activin on dog follicle development and survival, oocyte quality, and FSH receptor expression in culture. Preantral (150 - ≤230 μm diameter), early antral (231 - ≤330 μm), and antral (>330-550 μm) stage follicles were encapsulated in a fibrin-alginate h ...[more]

PMID: 30856402

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Project description:Regulation of human ovarian steroidogenesis differs from other species and precise knowledge on how human small antral follicles (hSAF) develop and acquire competence for continued growth and steroid output is still incomplete. The present study has characterized almost 1,000 normal hSAF collected in connection with cryopreservation of ovarian tissue for fertility preservation. The antral follicles (ranging from 3 to 13?mm) were generally aspirated from one ovary surgically removed during the natural cycle, and the follicular fluid (FF) and the granulosa cells (GC) were isolated and snap-frozen. In FF, the following hormones were measured: inhibin-B, inhibin-A, AMH, follistatin, PAPP-A, estradiol, progesterone, testosterone, and androstenedione. In GC, mRNA gene expressions using q-PCR were measured for the following genes: FSHR, AMH, CYP19, and AR. All samples in which one of the abovementioned parameters was measured were included, but typically multiple parameters were measured. Highly significant differences in concentration and follicular content in relation to follicular diameter were found for all measured hormones despite massive variability in-between follicles for any given diameter. The results demonstrate that profound changes take place in the hormonal microenvironment around follicular diameters of 8-11?mm corresponding to when follicular selection occurs. At this point, inhibin-B and inhibin-A showed distinct peaks concomitant with a significant reduction in both AMH protein and mRNA expression. Concentrations of inhibins, androgens, FSHR, and AR were intimately associated, and it is suggested that inhibin-B in combination with PAPP-A and thereby IGF2 activity exerts important paracrine signaling at follicular selection. At the same time upregulation of estradiol synthesis and CYP19 mRNA expression increased steroid output profoundly. Furthermore, the highly significant association between FSHR and AR mRNA gene expression enforces important functions of androgens in follicular development. Collectively, these data reintroduce the understanding of the follicular phase as two parted in which regulation of steroidogenesis differs. The profound changes taking place around follicular selection highlight important paracrine actions of TGF-? family members and IGFs for securing dominance of the selected follicle.

Dataset Information

Activin promotes growth and antral cavity expansion in the dog ovarian follicle.

Publications

Activin promotes growth and antral cavity expansion in the dog ovarian follicle.

Similar Datasets

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