Ontology highlight
ABSTRACT:
SUBMITTER: Ransom EM
PROVIDER: S-EPMC6446242 | biostudies-literature | 2018 Nov
REPOSITORIES: biostudies-literature
Ransom Eric M EM Kaus Gabriela M GM Tran Phuong M PM Ellermeier Craig D CD Weiss David S DS
Molecular microbiology 20181014 4
Clostridioides (formerly Clostridium) difficile produces two major toxins, TcdA and TcdB, upon entry into stationary phase. Transcription of tcdA and tcdB requires the specialized sigma factor, σ<sup>TcdR</sup> , which also directs RNA Polymerase to transcribe tcdR itself. We fused a gene for a red fluorescent protein to the tcdA promoter to study toxin gene expression at the level of individual C. difficile cells. Surprisingly, only a subset of cells became red fluorescent upon entry into stati ...[more]