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Histone H3.3 G34 Mutations Alter Histone H3K36 and H3K27 Methylation In Cis.

ABSTRACT: Histone H3 encoding genes, particularly H3F3A and H3F3B, the genes encoding the variant histone H3.3, are mutated at high frequency in pediatric brain and bone malignancies. Compared to the extensive studies on K27M and K36M mutations, little is known about the mechanism of G34 mutations found in pediatric glioblastoma or giant cell tumors of the bone. Here we report that unlike the K27M or K36M that affect global histone methylation, the giant cell tumors of the bone G34 mutations (G34L/W) only affect histone H3K36 and H3K27 methylation on the same mutated histone tails (in cis), a mechanism distinct from known histone mutations.

SUBMITTER: Shi L

PROVIDER: S-EPMC6450091 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature

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Histone H3.3 G34 Mutations Alter Histone H3K36 and H3K27 Methylation In Cis.

Shi Leilei L Shi Jiejun J Shi Xiaobing X Li Wei W Wen Hong H

Journal of molecular biology 20180422 11

Histone H3 encoding genes, particularly H3F3A and H3F3B, the genes encoding the variant histone H3.3, are mutated at high frequency in pediatric brain and bone malignancies. Compared to the extensive studies on K27M and K36M mutations, little is known about the mechanism of G34 mutations found in pediatric glioblastoma or giant cell tumors of the bone. Here we report that unlike the K27M or K36M that affect global histone methylation, the giant cell tumors of the bone G34 mutations (G34L/W) only ...[more]

PMID: 29689253

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Histone H3.3 G34 mutations alter histone H3K36 and H3K27 methylation in cis

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Histone H3.3 G34 mutations alter histone H3K36 and H3K27 methylation in cis

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Histone H3.3 G34 mutations promote aberrant PRC2 activity and drive tumor progression.

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