The In Vitro Evaluation of Povidone-Iodine Against Multiple Ocular Adenoviral Types.

ABSTRACT: PURPOSE:Povidone-iodine (P-I) is being touted as a topical antiviral treatment for eye infections caused by adenovirus (Ad). This study evaluated the in vitro antiviral activity of the several P-I concentrations previously used in clinical studies against multiple ocular Ad types commonly associated with eye infections. METHODS:The antiviral activity of four concentrations of P-I was compared to vehicle for seven types of Ad after incubating the P-I with Ad at 33°C for various lengths of time. Following incubation and neutralization of the P-I with sodium thiosulfate, viral titers were determined for each Ad type and time point. RESULTS:Virucidal (99.9%, ?3-Log10) reductions in titers were produced for 5%, 2%, and 0.4% P-I at 1?min for types Ad5 and Ad7a. Similar reductions were produced at 5?min for types Ad3, Ad4, and Ad8. For type Ad19/64, virucidal reductions took 60?min for 5% P-I and 15?min for 2% and 0.4%. For type Ad37, 60?min (5%), 15?min (2%), and 5?min (0.4%) were required to produce virucidal reductions. There were no virucidal reductions in titers produced by 0.001% P-I. CONCLUSIONS:P-I produced greater than 3-Log10 reductions of titers at 1-5?min for most of the ocular types tested (types Ad3, Ad4, Ad5, Ad7a, and Ad8). However, it took longer (15-60?min) for these reductions to be produced for types Ad19/64 and Ad37. The antiviral activity of P-I may be Ad type dependent.

SUBMITTER: Yates KA 

PROVIDER: S-EPMC6450451 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature