Ontology highlight
ABSTRACT:
SUBMITTER: Sengupta R
PROVIDER: S-EPMC6451417 | biostudies-literature | 2019 Mar
REPOSITORIES: biostudies-literature
Journal of cell science 20190318 6
We describe a method, termed cryoAPEX, which couples chemical fixation and high-pressure freezing of cells with peroxidase tagging (APEX) to allow precise localization of membrane proteins in the context of a well-preserved subcellular membrane architecture. Further, cryoAPEX is compatible with electron tomography. As an example, we apply cryoAPEX to obtain a high-resolution three-dimensional contextual map of the human FIC (filamentation induced by cAMP) protein, HYPE (also known as FICD). HYPE ...[more]