Dataset Information

Expression analysis data of T-cell surface antigen CD2 gene from rock bream (Oplegnathus fasciatus).

ABSTRACT: This data article reports the expression level of T-cell surface antigen CD2 gene in organs from normal rock bream through quantitative real-time PCR. We also report the expression level of CD2 gene when anthropogenic infection with bacterial or viral pathogens was induced. The expression pattern of CD2 gene in normal rock bream was proved to be highly expressed in hematopoietic cells involved in the production and development of peripheral blood leukocytes (PBLs) and immune cells. It also proved that it maintains high expression for normal immunity in gills, skin, and intestines exposed directly to the environment. The expression pattern of CD2 gene in pathogenic infection has proven that CD2 is a factor involved in immunity. These data are considered to be a basic study of teleost immune system and will contribute to the study of fish blood cells.

SUBMITTER: Cho DH

PROVIDER: S-EPMC6451685 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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Expression analysis data of T-cell surface antigen CD2 gene from rock bream (<i>Oplegnathus fasciatus</i>).

Cho Dong-Hee DH Kim Mu-Chan MC Park Chan-Il CI

Data in brief 20190326

This data article reports the expression level of T-cell surface antigen CD2 gene in organs from normal rock bream through quantitative real-time PCR. We also report the expression level of CD2 gene when anthropogenic infection with bacterial or viral pathogens was induced. The expression pattern of CD2 gene in normal rock bream was proved to be highly expressed in hematopoietic cells involved in the production and development of peripheral blood leukocytes (PBLs) and immune cells. It also prove ...[more]

PMID: 30997371

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Project description:Rock bream iridovirus (RBIV) is a notorious agent that causes high mortality in aquaculture of rock bream (Oplegnathus fasciatus). Despite severity of this virus, no transcriptomic studies on RBIV-infected rock bream that can provide fundamental information on protective mechanism against the virus have been reported so far. This study aimed to investigate physiological mechanisms between host and RBIV through transcriptomic changes in the spleen based on RNA-seq. Depending on infection intensity and sampling time point, fish were divided into five groups: uninfected healthy fish at week 0 as control (0C), heavy infected fish at week 0 (0H), heavy mixed RBIV and bacterial infected fish at week 0 (0MH), uninfected healthy fish at week 3 (3C), and light infected fish at week 3 (3L). We explored clusters from 35,861 genes with Fragments Per Kilo-base of exon per Million mapped fragments (FPKM) values of 0.01 or more through signed co-expression network analysis using WGCNA package. Nine of 22 modules were highly correlated with viral infection (|gene significance (GS) vs. module membership (MM) |> 0.5, p-value < 0.05). Expression patterns in selected modules were divided into two: heavy infected (0H and 0MH) and control and light-infected groups (0C, 3C, and 3L). In functional analysis, genes in two positive modules (5448 unigenes) were enriched in cell cycle, DNA replication, transcription, and translation, and increased glycolysis activity. Seven negative modules (3517 unigenes) built in this study showed significant decreases in the expression of genes in lymphocyte-mediated immune system, antigen presentation, and platelet activation, whereas there was significant increased expression of endogenous apoptosis-related genes. These changes lead to RBIV proliferation and failure of host defense, and suggests the importance of blood cells such as thrombocytes and B cells in rock bream in RBIV infection. Interestingly, a hub gene, pre-mRNA processing factor 19 (PRPF19) showing high connectivity (kME), and expression of this gene using qRT-PCR was increased in rock bream blood cells shortly after RBIV was added. It might be a potential biomarker for diagnosis and vaccine studies in rock bream against RBIV. This transcriptome approach and our findings provide new insight into the understanding of global rock bream-RBIV interactions including immune and pathogenesis mechanisms.

Dataset Information

Expression analysis data of T-cell surface antigen CD2 gene from rock bream (Oplegnathus fasciatus).

Publications

Expression analysis data of T-cell surface antigen CD2 gene from rock bream (<i>Oplegnathus fasciatus</i>).

Similar Datasets

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