Dataset Information

Human sapovirus GI.2 and GI.3 from children with acute gastroenteritis in northern Brazil.

ABSTRACT: Human sapoviruses (HSaV) are considered important causative agents of acute gastroenteritis in humans worldwide. However, knowledge of the genetic characteristics of the whole genome of HSaV in Brazil is limited. Here we report the complete genome sequences of six HSaVs GI.2 and two GI.3 strains obtained from children with acute gastroenteritis in the Northern region of Brazil. Next generation sequencing was used to obtain the full genome and molecular characterization of the genome was performed. Phylogenetic analysis of the genome was also performed. Only one complete HSaV GI.2 genome characterization in the country precedes that of the present study. This is the first complete genome sequence of genotype GI.3 in Brazil. The data obtained in this investigation can contribute to the augmentation of the database on the molecular diversity of HSaVs strains circulating in Brazil, and to the improvement of current typing protocols.

SUBMITTER: Cilli A

PROVIDER: S-EPMC6452520 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

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Human sapovirus GI.2 and GI.3 from children with acute gastroenteritis in northern Brazil.

Cilli Audrey A Luchs Adriana A Leal Elcio E Gill Danielle D Milagres Flavio Augusto de Pádua FAP Komninakis Shirley Vasconcelos SV Brustulin Rafael R Teles Maria da Aparecida Rodrigues MDAR Lobato Márcia Cristina Alves Brito Sayão MCABS Chagas Rogério Togisaki das RTD Abrão Maria de Fátima Neves Dos Santos MFNDS Soares Cassia Vitória de Deus Alves CVDA Deng Xutao X Delwart Eric E Sabino Ester Cerdeira EC Costa Antonio Charlys da ACD

Memorias do Instituto Oswaldo Cruz 20190404

Human sapoviruses (HSaV) are considered important causative agents of acute gastroenteritis in humans worldwide. However, knowledge of the genetic characteristics of the whole genome of HSaV in Brazil is limited. Here we report the complete genome sequences of six HSaVs GI.2 and two GI.3 strains obtained from children with acute gastroenteritis in the Northern region of Brazil. Next generation sequencing was used to obtain the full genome and molecular characterization of the genome was performe ...[more]

PMID: 30970051

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Project description:BackgroundRotavirus antigenemia and RNAemia (the presence of rotavirus RNA in serum) have been commonly identified among paediatric patients with acute gastroenteritis. In this study we examined the association between rotavirus antigenemia and clinical features, and sought to determine the genotypes of rotaviruses detected in paired stool and serum samples.MethodsPaired stool and serum samples were obtained from children hospitalised for acute gastroenteritis in Belém, Brazil, between June 2012 and June 2015. The 20-point Vesikari scoring system was used to assess the disease severity upon a retrospective medical record review. Stool and serum samples were primarily screened for the presence of rotavirus antigen using a commercial ELISA assay. The rotavirus isolates from stool and serum samples were genotyped by using the classical reverse-transcriptase polymerase chain reaction (RT-PCR) and/or through nucleotide sequencing of VP4 and VP7 genes. Viral load was estimated using real-time RT-PCR.ResultsIn total rotavirus antigen was detected in 109 (24.2%) stool samples from 451 children, whereas antigenemia occurred in 38.5% (42/109) of these patients. We demonstrated that patients positive for rotavirus RNA in paired stool and serum samples were more likely to have a higher frequency of vomiting episodes in a 24-h period (p = 0.0035). Our findings also suggested that children not vaccinated against rotavirus are more likely to develop antigenemia, as compared to those given at least one vaccine dose (p = 0.0151). G12P [8] and G2P [4] genotypes were predominant throughout the study period, accounting for 52.3% (57/109) and 27.5% (30/109) of the typed isolates, respectively. Ten stool-serum pairs could be typed for VP4 and VP7 genes. Seven of these pairs showed concordant results with G2P [4] genotype being detected in stool and serum samples, whereas discrepancies between genotypes (G2P [4]/G2P[NT] and G12P [8]/G2P[NT]) were seen in three pairs.ConclusionsRotavirus antigenemia and RNAemia occur in a significant number of children hospitalised for acute gastroenteritis in Belém, Brazil, and may contribute to a greater disease severity, particularly translated into a greater number of vomiting episodes. This study documented a high concordance of genotypes detected in a subgroup of paired stool and serum samples.

Dataset Information

Human sapovirus GI.2 and GI.3 from children with acute gastroenteritis in northern Brazil.

Publications

Human sapovirus GI.2 and GI.3 from children with acute gastroenteritis in northern Brazil.

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