Browse
Submit Data
Databases
API
Help

Dataset Information

8 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Surface Fluctuations and Coalescence of Nucleolar Droplets in the Human Cell Nucleus.

ABSTRACT: The nucleolus is a membraneless organelle embedded in chromatin solution inside the cell nucleus. By analyzing surface dynamics and fusion kinetics of human nucleoli in vivo, we find that the nucleolar surface exhibits subtle, but measurable, shape fluctuations and that the radius of the neck connecting two fusing nucleoli grows in time as r(t)?t^{1/2}. This is consistent with liquid droplets with low surface tension ?10^{-6}??N?m^{-1} coalescing within an outside fluid of high viscosity ?10^{3}??Pa?s. Our study presents a noninvasive approach of using natural probes and their dynamics to investigate material properties of the cell and its constituents.

SUBMITTER: Caragine CM

PROVIDER: S-EPMC6452643 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Publications

Surface Fluctuations and Coalescence of Nucleolar Droplets in the Human Cell Nucleus.

Caragine Christina M CM Haley Shannon C SC Zidovska Alexandra A

Physical review letters 20181001 14

The nucleolus is a membraneless organelle embedded in chromatin solution inside the cell nucleus. By analyzing surface dynamics and fusion kinetics of human nucleoli in vivo, we find that the nucleolar surface exhibits subtle, but measurable, shape fluctuations and that the radius of the neck connecting two fusing nucleoli grows in time as r(t)∼t^{1/2}. This is consistent with liquid droplets with low surface tension ∼10^{-6} N m^{-1} coalescing within an outside fluid of high viscosity ∼10^{3} ...[more]

PMID: 30339413

Similar Datasets

Temperature-induced coalescence of colliding binary droplets on superhydrophobic surface.

Project description:This report investigates the impact of droplet temperature on the head-on collision of binary droplets on a superhydrophobic surface. Understanding droplet collision is critical to many fundamental processes and industrial applications. There are many factors, including collision speed, collision angle, and droplet composition, that influence the outcome of the collision between binary droplets. This work provides the first experimental study of the influence of droplet temperature on the collision of binary droplets. As the droplet temperature increases, the possibility increases for the two droplets to coalesce after collision. The findings in this study can be extended to collision of droplets under other conditions where control of the droplet temperature is feasible. Such findings will also be beneficial to applications that involve droplet collision, such as in ink-jet printing, steam turbines, engine ignition, and spraying cooling.

| S-EPMC3946014 | biostudies-other

Coalescence Dynamics of Acoustically Levitated Droplets.

Project description:The contactless coalescence of a droplet is of paramount importance for physical and industrial applications. This paper describes a coalescence method to be used mid-air via acoustic levitation using an ultrasonic phased array system. Acoustic levitation using ultrasonic phased arrays provides promising lab-on-a-drop applications, such as transportation, coalescence, mixing, separation, evaporation, and extraction in a continuous operation. The mechanism of droplet coalescence in mid-air may be better understood by experimentally and numerically exploring the droplet dynamics immediately before the coalescence. In this study, water droplets were experimentally levitated, transported, and coalesced by controlled acoustic fields. We observed that the edges of droplets deformed and attracted each other immediately before the coalescence. Through image processing, the radii of curvature of the droplets were quantified and the pressure difference between the inside and outside a droplet was simulated to obtain the pressure and velocity information on the droplet's surface. The results revealed that the sound pressure acting on the droplet clearly decreased before the impact of the droplets. This pressure on the droplets was quantitatively analyzed from the experimental data. Our experimental and numerical results provide deeper physical insights into contactless droplet manipulation for futuristic lab-on-a-drop applications.

| S-EPMC7231308 | biostudies-literature

Coalescence, Spreading, and Rebound of Two Water Droplets with Different Temperatures on a Superhydrophobic Surface.

Project description:This paper studied the coalescence, spreading, and rebound of two droplets with different temperatures on a superhydrophobic surface. When the temperature of the impacting droplet was the same as that of the stationary droplet, there was a large deformation of both droplets before the coalescence and the energy dissipation was also large. The coalescence happened at the time close to the maximum spreading. When the temperature of the impacting droplet increased, the deformation of both droplets became smaller before the coalescence and the coalescence happened at or even before the droplets started to spread. The energy dissipation and loss in the later situation is less than those in the previous case. The rebounding characteristics of the merged droplets were also found to be dependent on the temperature. There is an optimum temperature at which the merged droplets can rebound for more times due to the balance of energy loss and also the interaction of the merged droplets with the underlying superhydrophobic substrate. These findings may help further the fundamental understanding of droplet collision on a superhydrophobic surfaces and also offer an alternative strategy to remove droplets from the underlying surfaces for different industrial applications, including condensation heat transfer in steam power plants and phase-change-based thermal management systems.

| S-EPMC6822121 | biostudies-literature

Coalescence and breakup of oppositely charged droplets.

Project description:The coalescence process of oppositely charged drops for different electrical conductivities of liquids is presented. When the electrical conductivity was relatively low, oppositely charged drops failed to coalesce under sufficiently high electrical fields and capillary ripples were formed on the surfaces of droplets after rebound. For a high electrically conductive liquid, it was found that a crown profile of drop fission always appeared on the top surface of negatively charged drops after the two charged drops contacted and bounced off. Furthermore, we report here, for the first time, the newly found phenomenon and argue that the break up might be caused by Rayleigh instability, a form of Coulomb fission. The different mobility of positive and negative ions is the underlying mechanism that explains why the break up always happened on the negative side of charged drops.

| S-EPMC4238017 | biostudies-other

Non-coalescence of oppositely charged droplets in pH-sensitive emulsions.

Project description:Like charges stabilize emulsions, whereas opposite charges break emulsions. This is the fundamental principle for many industrial and practical processes. Using micrometer-sized pH-sensitive polymeric hydrogel particles as emulsion stabilizers, we prepare emulsions that consist of oppositely charged droplets, which do not coalesce. We observe noncoalescence of oppositely charged droplets in bulk emulsification as well as in microfluidic devices, where oppositely charged droplets are forced to collide within channel junctions. The results demonstrate that electrostatic interactions between droplets do not determine their stability and reveal the unique pH-dependent properties of emulsions stabilized by soft microgel particles. The noncoalescence can be switched to coalescence by neutralizing the microgels, and the emulsion can be broken on demand. This unusual feature of the microgel-stabilized emulsions offers fascinating opportunities for future applications of these systems.

| S-EPMC3258642 | biostudies-literature

Universality in the viscous-to-inertial coalescence of liquid droplets.

Project description:We present a theory on the coalescence of 2 spherical liquid droplets that are initially stationary. The evolution of the radius of a liquid neck formed upon coalescence was formulated as an initial value problem and then solved to yield an exact solution without free parameters, with its 2 asymptotic approximations reproducing the well-known scaling relations in the inertially limited viscous and inertial regimes. The viscous-to-inertial crossover observed in previous research is also recovered by the theory, rendering the collapse of data of different viscosities onto a single curve.

| S-EPMC6876233 | biostudies-literature

Coalescence-induced jumping of droplets on superomniphobic surfaces with macrotexture.

Project description:When two liquid droplets coalesce on a superrepellent surface, the excess surface energy is partly converted to upward kinetic energy, and the coalesced droplet jumps away from the surface. However, the efficiency of this energy conversion is very low. In this work, we used a simple and passive technique consisting of superomniphobic surfaces with a macrotexture (comparable to the droplet size) to experimentally demonstrate coalescence-induced jumping with an energy conversion efficiency of 18.8% (i.e., about 570% increase compared to superomniphobic surfaces without a macrotexture). The higher energy conversion efficiency arises primarily from the effective redirection of in-plane velocity vectors to out-of-plane velocity vectors by the macrotexture. Using this higher energy conversion efficiency, we demonstrated coalescence-induced jumping of droplets with low surface tension (26.6 mN m-1) and very high viscosity (220 mPa·s). These results constitute the first-ever demonstration of coalescence-induced jumping of droplets at Ohnesorge number >1.

| S-EPMC6226286 | biostudies-literature

Wettability and Coalescence of Cu Droplets Subjected to Two-Wall Confinement.

Project description:Controlling droplet dynamics via wettability or movement at the nanoscale is a significant goal of nanotechnology. By performing molecular dynamics simulations, we study the wettability and spontaneous coalescence of Cu droplets confined in two carbon walls. We first focus on one drop in the two-wall confinement to reveal confinement effects on wettability and detaching behavior of metallic droplets. Results show that Cu droplets finally display three states: non-detachment, semi-detachment and full detachment, depending on the height of confined space. The contact angle ranges from 125° to 177°, and the contact area radius ranges from 12 to ~80 Å. The moving time of the detached droplet in the full detachment state shows a linear relationship with the height of confined space. Further investigations into two drops subjected to confinement show that the droplets, initially distant from each other, spontaneously coalesce into a larger droplet by detachment. The coalescing time and final position of the merged droplet are precisely controlled by tailoring surface structures of the carbon walls, the height of the confined space or a combination of these approaches. These findings could provide an effective method to control the droplet dynamics by confinement.

| S-EPMC4602311 | biostudies-literature

Coalescence-Induced Jumping of Multiple Condensate Droplets on Hierarchical Superhydrophobic Surfaces.

Project description:Coalescence-induced jumping of condensate droplets from a superhydrophobic surface with hierarchical micro/nanoscale roughness is quantitatively characterized. Experimental observations show that the condensate droplet jumping is induced by coalescence of multiple droplets of different sizes, and that the coalesced droplet trajectories typically deviate from the surface normal. A depth-from-defocus image processing technique is developed to track the out-of-plane displacement of the jumping droplets, so as to accurately measure the droplet size and velocity. The results demonstrate that the highest jumping velocity is achieved when two droplets coalesce. The jumping velocity decreases gradually with an increase in the number of coalescing droplets, despite the greater potential surface energy released upon coalescence. A general theoretical model that accounts for viscous dissipation, surface adhesion, line tension, the initial droplet wetting states, and the number and sizes of the coalescing droplets is developed to explain the trends of droplet jumping velocity observed in the experiments.

| S-EPMC4698694 | biostudies-literature

Optically controlled coalescence and splitting of femtoliter/picoliter droplets for microreactors.

Project description:Microreactor technology has attracted tremendous interest due to its features of a large specific surface area, low consumption of reagents and energy, and flexible control of the reaction process. As most of the current microreactors have volumes of microliters or even larger, effective methods to reduce the microreactors' sizes and improve their flexibility and controllability have become highly demanded. Here we propose an optical method of coalescence and splitting of femto-/pico-liter droplets for application in microreactors. Firstly, two different schemes are adopted to stably trap and directionally transport the microdroplets (oil and water) by a scanning optical tweezing system. Then, optically controlled coalescence and splitting of the microdroplets are achieved on this basis, and the mechanism and conditions are explored. Finally, the microdroplets are used as microreactors to conduct the microreactions. Such microreactors combine the advantages of miniaturization and the multi-functions of microdroplets, as well as the precision, flexibility, and non-invasiveness of optical tweezers, holding great potential for applications in materials synthesis and biosensing.

| S-EPMC9215165 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data