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Structural and dynamical rationale for fatty acid unsaturation in Escherichia coli.


ABSTRACT: Fatty acid biosynthesis in ?- and ?-proteobacteria requires two functionally distinct dehydratases, FabA and FabZ. Here, mechanistic cross-linking facilitates the structural characterization of a stable hexameric complex of six Escherichia coli FabZ dehydratase subunits with six AcpP acyl carrier proteins. The crystal structure sheds light on the divergent substrate selectivity of FabA and FabZ by revealing distinct architectures of the binding pocket. Molecular dynamics simulations demonstrate differential biasing of substrate orientations and conformations within the active sites of FabA and FabZ such that FabZ is preorganized to catalyze only dehydration, while FabA is primed for both dehydration and isomerization.

SUBMITTER: Dodge GJ 

PROVIDER: S-EPMC6452729 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

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Structural and dynamical rationale for fatty acid unsaturation in <i>Escherichia coli</i>.

Dodge Greg J GJ   Patel Ashay A   Jaremko Kara L KL   McCammon J Andrew JA   Smith Janet L JL   Burkart Michael D MD  

Proceedings of the National Academy of Sciences of the United States of America 20190314 14


Fatty acid biosynthesis in α- and γ-proteobacteria requires two functionally distinct dehydratases, FabA and FabZ. Here, mechanistic cross-linking facilitates the structural characterization of a stable hexameric complex of six <i>Escherichia coli</i> FabZ dehydratase subunits with six AcpP acyl carrier proteins. The crystal structure sheds light on the divergent substrate selectivity of FabA and FabZ by revealing distinct architectures of the binding pocket. Molecular dynamics simulations demon  ...[more]

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