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Optimization of solvent-free enzymatic esterification in eutectic substrate reaction mixture.


ABSTRACT: The Candida rugosa lipase catalyzed esterification of (-)-menthol and lauric acid (LA) was studied in a eutectic mixture formed by both substrates((-)-menthol:LA 3:1, mol/mol). No additional reaction solvent was necessary, since the (-)-menthol:LA deep eutectic solvent (DES) acts as combined reaction medium and substrate pool. Therefore, the esterification is conducted under solvent-free conditions. The thermodynamic water activity (aw) was identified as a key parameter affecting the esterification performance in the (-)-menthol:LA DES. A response surface methodology was applied to optimize the esterification conditions in terms aw, amount of C. rugosa lipase (mCRL) and reaction temperature. Under the optimized reaction conditions (aw?=?0.55; mCRL =60?mg; T =45?°C), a conversion of 95?±?1% LA was achieved (one day), the final (-)-menthyl lauric acid ester concentration reached 1.36?±?0.04?M (2.25 days). The experimental product formation rate agreed very well with the model prediction.

SUBMITTER: Patzold M 

PROVIDER: S-EPMC6453777 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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Optimization of solvent-free enzymatic esterification in eutectic substrate reaction mixture.

Pätzold M M   Weimer A A   Liese A A   Holtmann D D  

Biotechnology reports (Amsterdam, Netherlands) 20190402


The <i>Candida rugosa</i> lipase catalyzed esterification of (-)-menthol and lauric acid (LA) was studied in a eutectic mixture formed by both substrates((-)-menthol:LA 3:1, mol/mol). No additional reaction solvent was necessary, since the (-)-menthol:LA deep eutectic solvent (DES) acts as combined reaction medium and substrate pool. Therefore, the esterification is conducted under solvent-free conditions. The thermodynamic water activity (a<sub>w</sub>) was identified as a key parameter affecti  ...[more]

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