Comparative analysis of sequencing technologies for single-cell transcriptomics.
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ABSTRACT:
SUBMITTER: Natarajan KN
PROVIDER: S-EPMC6454680 | biostudies-literature | 2019 Apr
REPOSITORIES: biostudies-literature
Publications
Comparative analysis of sequencing technologies for single-cell transcriptomics.
Natarajan Kedar Nath KN Miao Zhichao Z Jiang Miaomiao M Huang Xiaoyun X Zhou Hongpo H Xie Jiarui J Wang Chunqing C Qin Shishang S Zhao Zhikun Z Wu Liang L Yang Naibo N Li Bo B Hou Yong Y Liu Shiping S Teichmann Sarah A SA
Genome biology 20190409 1
Single-cell RNA-seq technologies require library preparation prior to sequencing. Here, we present the first report to compare the cheaper BGISEQ-500 platform to the Illumina HiSeq platform for scRNA-seq. We generate a resource of 468 single cells and 1297 matched single cDNA samples, performing SMARTer and Smart-seq2 protocols on two cell lines with RNA spike-ins. We sequence these libraries on both platforms using single- and paired-end reads. The platforms have comparable sensitivity and accu ...[more]
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