Selenium Supplementation Alters Hepatic Energy and Fatty Acid Metabolism in Mice.

ABSTRACT:

Background

Human and animal studies have raised concerns that supplemental selenium can increase the risk of metabolic disorders, but underlying mechanisms are unclear.

Objective

We used an integrated transcriptome and metabolome analysis of liver to test for functional pathway and network responses to supplemental selenium in mice.

Methods

Male mice (8-wk-old, C57BL/6J) fed a standard diet (0.41 ppm Se) were given selenium (Na2SeO4, 20 μmol/L) or vehicle (drinking water) for 16 wk. Livers were analyzed for selenium concentration, activity of selenoproteins, reduced glutathione (GSH) redox state, gene expression, and high-resolution metabolomics. Transcriptomic and nontargeted metabolomic data were analyzed with biostatistics, bioinformatics, pathway enrichment analys

SUBMITTER: Hu X 

PROVIDER: S-EPMC6454983 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature