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Complete Genome Sequence of an Ehrlichia minasensis Strain Isolated from Cattle.

ABSTRACT: Ehrlichia minasensis is a tick-borne pathogen affecting cattle, cervids, and dogs, and it is closely related to the monocytotropic pathogen Ehrlichia canis Here, we announce the draft genome sequence of Ehrlichia minasensis strain Cuiabá, isolated from a naturally infected calf from Santo Antônio do Leverger, Mato Grosso, Brazil.

SUBMITTER: Aguiar DM

PROVIDER: S-EPMC6460032 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

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Complete Genome Sequence of an Ehrlichia minasensis Strain Isolated from Cattle.

Aguiar Daniel M DM Araujo João P JP Nakazato Luciano L Bard Emilie E Cabezas-Cruz Alejandro A

Microbiology resource announcements 20190411 15

Ehrlichia minasensis is a tick-borne pathogen affecting cattle, cervids, and dogs, and it is closely related to the monocytotropic pathogen Ehrlichia canis Here, we announce the draft genome sequence of Ehrlichia minasensis strain Cuiabá, isolated from a naturally infected calf from Santo Antônio do Leverger, Mato Grosso, Brazil. ...[more]

PMID: 30975809

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Project description:The genus Ehrlichia is composed of tick-borne obligate intracellular gram-negative alphaproteobacteria of the family Anaplasmataceae. Ehrlichia includes important pathogens affecting canids (E. canis, E. chaffeensis, and E. ewingii), rodents (E. muris), and ruminants (E. ruminantium). Ehrlichiaminasensis, an Ehrlichia closely related to E. canis, was initially reported in Canada and Brazil. This bacterium has now been reported in Pakistan, Malaysia, China, Ethiopia, South Africa, and the Mediterranean island of Corsica, suggesting that E. minasensis has a wide geographical distribution. Previously, E. minasensis was found to cause clinical ehrlichiosis in an experimentally infected calf. The type strain E. minasensis UFMG-EV was successfully isolated from Rhipicephalus microplus ticks and propagated in the tick embryonic cell line of Ixodes scapularis (IDE8). However, the isolation and propagation of E. minasensis strains from cattle has remained elusive. In this study, the E. minasensis strain Cuiabá was isolated from an eight-month-old male calf of Holstein breed that was naturally infected with the bacterium. The calf presented clinical signs and hematological parameters of bovine ehrlichiosis. The in vitro culture of the agent was established in the canine cell line DH82. Ehrlichial morulae were observed using light and electron microscopy within DH82 cells. Total DNA was extracted, and the full genome of the E. minasensis strain Cuiabá was sequenced. A core-genome-based phylogenetic tree of Ehrlichia spp. and Anaplasma spp. confirmed that E. minasensis is a sister taxa of E. canis. A comparison of functional categories among Ehrlichia showed that E. minasensis has significantly less genes in the 'clustering-based subsystems' category, which includes functionally coupled genes for which the functional attributes are not well understood. Results strongly suggest that E. minasensis is a novel pathogen infecting cattle. The epidemiology of this Ehrlichia deserves further attention because these bacteria could be an overlooked cause of tick-borne bovine ehrlichiosis, with a wide distribution.

Dataset Information

Complete Genome Sequence of an Ehrlichia minasensis Strain Isolated from Cattle.

Publications

Complete Genome Sequence of an <i>Ehrlichia minasensis</i> Strain Isolated from Cattle.

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