25(OH)D3 and 1.25(OH)2D3 inhibits TNF-? expression in human monocyte derived macrophages.
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ABSTRACT: Purpose
We wanted to investigate effects of vitamin D3 (25(OH)D3 and 1.25(OH)2D3) on inflammatory cytokine expression in both activated and non-activated M?.Materials and methods
Mononuclear cells, isolated from healthy donor buffy coats were cultured for a 6-day differentiation-period. Fully differentiated M? were pre-treated with either 25(OH)D3 or 1.25(OH)2D3 for (4, 12 or 24 hours) +/-LPS challenge for 4 hours. Gene expression analyses of VDR, Cyp27b1 and pro-inflammatory markers TNF-?, IL-6, NF-?B, MCP-1, was performed using RT-quantitative PCR. TNF-? protein levels from M? culture media were analysed by ELISA.Results
Both 25(OH)D3 and 1.25(OH)2D3 significantly inhibited TNF-? expression in both LPS-stimulated and unstimulated M?. Also, NF-?B, and to a lesser extend IL-6 and MCP-1 were inhibited. LPS up-regulated Cyp27b1 gene expression which was partly reverted by 1.25(OH)2D3.Conclusion
These data show anti-inflammatory effects of vitamin D3 (25(OH)D3 and 1.25(OH)2D3) in human macrophages, and support, that means for targeting high dose vitamin D3 to the immune system may have beneficial clinical effect in inflammatory conditions.
SUBMITTER: Rafique A
PROVIDER: S-EPMC6461260 | biostudies-literature | 2019
REPOSITORIES: biostudies-literature
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