Dataset Information

Production and evaluation of mono- and di-rhamnolipids produced by Pseudomonas aeruginosa VM011.

ABSTRACT: Rhamnolipids are amphiphilic compounds secreted by bacteria and possess the emulsification ability. Emulsification ability makes microbial surfactants an excellent candidate for assisting in the breakdown and removal of oil spills. Rhamnolipids have been demonstrated for their antibacterial and antifungal activities. This suggests that rhamnolipids play vital roles in the medical, agricultural, bioremediation etc. In the present study, bacterial strain VM011 was isolated from organic farm soil, located nearby Zuari River in Durbhat (Goa, India), where farmlands were irrigated by borewell water. Isolated bacterial strain VM011 was identified as Pseudomonas aeruginosa per the Bergey's Manual of Systematic Bacteriology. The Rhamnolipid production ability of Pseudomonas aeruginosa VM011 was confirmed using NaCl-methylene blue agar method. Furthermore, rhamnolipid produced by P. aeruginosa VM011 emulsify the combustible hydrocarbon such as kerosene (lamp oil). As produced rhamnolipids has an oil-like appearance and consists of two different rhamnolipid confirmed by thin layer chromatography data-di-rhamnolipid with Rf value = 0.16 and mono-rhamnolipid with Rf value = 0.37.

SUBMITTER: Rekadwad B

PROVIDER: S-EPMC6461592 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Production and evaluation of mono- and di-rhamnolipids produced by <i>Pseudomonas aeruginosa</i> VM011.

Rekadwad Bhagwan B Maske Vikas V Khobragade Chandrahasya N CN Kasbe Prajapati S PS

Data in brief 20190403

Rhamnolipids are amphiphilic compounds secreted by bacteria and possess the emulsification ability. Emulsification ability makes microbial surfactants an excellent candidate for assisting in the breakdown and removal of oil spills. Rhamnolipids have been demonstrated for their antibacterial and antifungal activities. This suggests that rhamnolipids play vital roles in the medical, agricultural, bioremediation etc. In the present study, bacterial strain VM011 was isolated from organic farm soil, ...[more]

PMID: 31011602

Similar Datasets

Project description:BACKGROUND:Rhamnolipids are the most extensively studied biosurfactants and has been successfully used in various areas from bioremediation to industrial fields. Rhamnolipids structural composition decide their physicochemical properties. Different physicochemical properties influence their application potential. Rhamnolipids can be produced at both aerobic conditions and anaerobic conditions by Pseudomonas aeruginosa. This study aims to evaluate the oxygen effects on the rhamnolipids yield, structural composition, physicochemical properties and the rhl-genes expression in P. aeruginosa SG. Results will guide researchers to regulate microbial cells to synthesize rhamnolipids with different activity according to diverse application requirements. RESULTS:Quantitative real-time PCR analysis revealed that rhlAB genes were down-regulated under anaerobic conditions. Therefore, strain P. aeruginosa SG anaerobically produced less rhamnolipids (0.68 g/L) than that (11.65 g/L) under aerobic conditions when grown in media containing glycerol and nitrate. HPLC-MS analysis showed that aerobically produced rhamnolipids mainly contained Rha-C8-C10, Rha-Rha-C10-C12:1 and Rha-Rha-C8-C10; anaerobically produced rhamnolipids mainly contained Rha-C10-C12 and Rha-C10-C10. Anaerobically produced rhamnolipids contained more mono-rhamnolipids (94.7%) than that (54.8%) in aerobically produced rhamnolipids. rhlC gene was also down-regulated under anaerobic conditions, catalyzing less mono-rhamnolipids to form di-rhamnolipids. Aerobically produced rhamnolipids decreased air-water surface tension (ST) from 72.2 to 27.9 mN/m with critical micelle concentration (CMC) of 60 mg/L; anaerobically produced rhamnolipids reduced ST to 33.1 mN/m with CMC of 80 mg/L. Anaerobically produced rhamnolipids emulsified crude oil with EI24 = 80.3%, and aerobically produced rhamnolipids emulsified crude oil with EI24 = 62.3%. Both two rhamnolipids products retained surface activity (ST < 35.0 mN/m) and emulsifying activity (EI24 > 60.0%) under temperatures (4-121 °C), pH values (4-10) and NaCl concentrations less than 90 g/L. CONCLUSIONS:Oxygen affected the rhl-genes expression in P. aeruginosa, thus altering the rhamnolipids yield, structural composition and physicochemical properties. Rhamnolipids produced at aerobic or anaerobic conditions was structurally distinct. Two rhamnolipids products had different application potential in diverse biotechnologies. Although both rhamnolipids products were thermo-stable and halo-tolerant, aerobically produced rhamnolipids possessed better surface activity, implying its well wetting activity and desorption property; anaerobically produced rhamnolipids exhibited better emulsifying activity, indicating its applicability for enhanced oil recovery and bioremediation of petroleum pollution.

Dataset Information

Production and evaluation of mono- and di-rhamnolipids produced by Pseudomonas aeruginosa VM011.

Publications

Production and evaluation of mono- and di-rhamnolipids produced by <i>Pseudomonas aeruginosa</i> VM011.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets