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Data on the absorbance of glucose during the acid hydrolysis of the sugarcane bagasse.

ABSTRACT: This article presents data relating to the changes in absorbance of glucose during the acid hydrolysis of sugarcane bagasse using sulphuric acid. This dataset also contains the moisture content, volatile matter, and fixed carbon of the sugarcane bagasse. The results of the analysis of variance (ANOVA) and the interaction plots between reaction time, temperature, and ratio are also presented. The data revealed that absorbance of glucose is increasing by increasing the temperature and time. Moreover, the best ratio for the highest absorbance of glucose was achieved at 1:20.

SUBMITTER: Alkarkhi AFM

PROVIDER: S-EPMC6461596 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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Data on the absorbance of glucose during the acid hydrolysis of the sugarcane bagasse.

Alkarkhi Abbas F M AFM Alqaraghuli Wasin A A WAA Yusup Yusri Y Abu Amr Salem S SS Mahmud M N MN Dewayantoa Nugroho N

Data in brief 20190403

This article presents data relating to the changes in absorbance of glucose during the acid hydrolysis of sugarcane bagasse using sulphuric acid. This dataset also contains the moisture content, volatile matter, and fixed carbon of the sugarcane bagasse. The results of the analysis of variance (ANOVA) and the interaction plots between reaction time, temperature, and ratio are also presented. The data revealed that absorbance of glucose is increasing by increasing the temperature and time. Moreov ...[more]

PMID: 31011604

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Project description:BACKGROUND:Most ?-glucosidases reported are sensitive to the end product (glucose), making it the rate limiting component of cellulase for efficient degradation of cellulose through enzymatic route. Thus, there are ongoing interests in searching for glucose-tolerant ?-glucosidases, which are still active at high glucose concentration. Although many ?-glucosidases with different glucose-tolerance levels have been isolated and characterized in the past decades, the effects of glucose-tolerance on the hydrolysis of cellulose are not thoroughly studied. RESULTS:In the present study, a novel ?-glucosidase (Bgl6) with the half maximal inhibitory concentration (IC 50) of 3.5 M glucose was isolated from a metagenomic library and characterized. However, its poor thermostability at 50 °C hindered the employment in cellulose hydrolysis. To improve its thermostability, random mutagenesis was performed. A thermostable mutant, M3, with three amino acid substitutions was obtained. The half-life of M3 at 50 °C is 48 h, while that of Bgl6 is 1 h. The K cat/K m value of M3 is 3-fold higher than that of Bgl6. The mutations maintained its high glucose-tolerance with IC 50 of 3.0 M for M3. In a 10-h hydrolysis of cellobiose, M3 completely converted cellobiose to glucose, while Bgl6 reached a conversion of 80 %. Then their synergistic effects with the commercial cellulase (Celluclast 1.5 L) on hydrolyzing pretreated sugarcane bagasse (SCB) were investigated. The supplementation of Bgl6 or mutant M3 to Celluclast 1.5 L significantly improved the SCB conversion from 64 % (Celluclast 1.5 L alone) to 79 % (Bgl6) and 94 % (M3), respectively. To further evaluate the application potential of M3 in high-solids cellulose hydrolysis, such reactions were performed at initial glucose concentration of 20-500 mM. Results showed that the supplementation of mutant M3 enhanced the glucose production from SCB under all the conditions tested, improving the SCB conversion by 14-35 %. CONCLUSIONS:These results not only clearly revealed the significant role of glucose-tolerance in cellulose hydrolysis, but also showed that mutant M3 may be a potent candidate for high-solids cellulose refining.

Dataset Information

Data on the absorbance of glucose during the acid hydrolysis of the sugarcane bagasse.

Publications

Data on the absorbance of glucose during the acid hydrolysis of the sugarcane bagasse.

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