Ontology highlight
ABSTRACT:
SUBMITTER: Sabantsev A
PROVIDER: S-EPMC6461674 | biostudies-literature | 2019 Apr
REPOSITORIES: biostudies-literature
Sabantsev Anton A Levendosky Robert F RF Zhuang Xiaowei X Bowman Gregory D GD Deindl Sebastian S
Nature communications 20190412 1
ATP-dependent chromatin remodelling enzymes (remodellers) regulate DNA accessibility in eukaryotic genomes. Many remodellers reposition (slide) nucleosomes, however, how DNA is propagated around the histone octamer during this process is unclear. Here we examine the real-time coordination of remodeller-induced DNA movements on both sides of the nucleosome using three-colour single-molecule FRET. During sliding by Chd1 and SNF2h remodellers, DNA is shifted discontinuously, with movement of entry- ...[more]