Ontology highlight
ABSTRACT:
SUBMITTER: Lim CC
PROVIDER: S-EPMC6465254 | biostudies-literature | 2019 Apr
REPOSITORIES: biostudies-literature
Lim Chia Chiu CC Woo Patrick C Y PCY Lim Theam Soon TS
Scientific reports 20190415 1
Antibody phage display has been pivotal in the quest to generate human monoclonal antibodies for biomedical and research applications. Target antigen preparation is a main bottleneck associated with the panning process. This includes production complexity, downstream purification, quality and yield. In many instances, purified antigens are preferred for panning but this may not be possible for certain difficult target antigens. Here, we describe an improved procedure of affinity selection agains ...[more]