Ontology highlight
ABSTRACT:
SUBMITTER: Pritchard RB
PROVIDER: S-EPMC6465260 | biostudies-literature | 2019 Apr
REPOSITORIES: biostudies-literature
Pritchard Ruth B RB Hansen D Flemming DF
Nature communications 20190415 1
Side chains cover protein surfaces and are fundamental to processes as diverse as substrate recognition, protein folding and enzyme catalysis. However, characterisation of side-chain motions has so far been restricted to small proteins and methyl-bearing side chains. Here we present a class of methods, based on <sup>13</sup>C-detected NMR spectroscopy, to more generally quantify motions and interactions of side chains in medium-to-large proteins. A single, uniformly isotopically labelled sample ...[more]